Π₯Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΌΠ΅ΡΠΈΠ»-ΠΠΠ ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ POZ-Π΄ΠΎΠΌΠ΅Π½ ΠΈ ΡΠΈΠ½ΠΊΠΎΠ²ΡΠ΅ ΠΏΠ°Π»ΡΡΡ
ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ZBTB4 ΠΈ ZBTB38 ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΡΡ Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠΉ ΠΠΠ in vivo. ΠΠ°Π½Π½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΠΏΡΠΈΠ²Π»Π΅ΠΊΠ°ΡΡΡΡ ΠΊ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΠΌ Ρ ΡΠΎΠΌΠΎΡΠ΅Π½ΡΡΠ°ΠΌ ΠΈ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΡΡΡ Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΠΌ DMR Π»ΠΎΠΊΡΡΠ° H19/IGF2- ΠΏΡΠΈ ΠΎΡΡΡΡΡΡΠ²ΠΈΠΈ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ dnmtl-/- Π΄Π°Π½Π½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΡΠΆΠ΅ Π½Π΅ ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΡΡ Ρ Ρ ΡΠΎΠΌΠΎΡΠ΅Π½ΡΡΠ°ΠΌΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ZBTB4 ΠΈ ZBTB38 ΡΠ²Π»ΡΡΡΡΡ ΠΌΠ΅ΡΠΈΠ»ΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΠΌΠΈ ΡΠ΅ΠΏΡΠ΅ΡΡΠΎΡΠ°ΠΌΠΈ, ΠΏΡΠΈΡΠ΅ΠΌ Π΄Π»Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅ΠΏΡΠ΅ΡΡΠΈΠΈ ZBTB4 Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
Π₯Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΌΠ΅ΡΠΈΠ»-ΠΠΠ ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ POZ-Π΄ΠΎΠΌΠ΅Π½ ΠΈ ΡΠΈΠ½ΠΊΠΎΠ²ΡΠ΅ ΠΏΠ°Π»ΡΡΡ (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- 1. 1. ΠΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π³Π΅Π½ΠΎΠΌΠ° ΡΡΠΊΠ°ΡΠΈΠΎΡ ΠΈ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ
- 1. 2. ΠΠΠ ΠΌΠ΅ΡΠΈΠ»ΡΡΠ°Π½ΡΡΠ΅ΡΠ°Π·Ρ. ΠΠ°ΡΠΈΡΠ° ΠΎΡ de novo ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ
- 1. 3. X ΠΈΠ½Π°ΠΊΡΠΈΠ²Π°ΡΠΈΡ. ΠΠ΅Π½ΠΎΠΌΠ½ΡΠΉ ΠΈΠΌΠΏΡΠΈΠ½ΡΠΈΠ½Π³
- 1. 4. ΠΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠΠ ΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ
- 1. 5. ΠΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π³ΠΈΡΡΠΎΠ½ΠΎΠ². Π‘Π²ΡΠ·Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΠΠ ΠΈ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠΉ Π³ΠΈΡΡΠΎΠ½ΠΎΠ²
- 1. 6. ΠΠ΅ΡΠΈΠ»-ΠΠΠ-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΠ΅ Π±Π΅Π»ΠΊΠΈ
- 1. 7. ΠΡΠ°ΡΠΊΠ°Ρ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° Π±Π΅Π»ΠΊΠΎΠ² ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° BTB/POZ
- 2. 1. 3. ΠΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΠΈΡΠΈΠ΄Ρ Π΄Π»Ρ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠ»Π½ΠΎΡΠ°Π·ΠΌΠ΅ΡΠ½ΡΡ
Kaiso, ZBTB4, ZBTB ΠΈ ΡΠΈΠ½ΠΊΠΎΠ²ΡΡ
ΠΏΠ°Π»ΡΡΠ΅Π² ZBTB4 ΠΈ ZBTB38 Π΄Π»Ρ ΠΈΠΌΡΠ½Π½ΠΎΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠΈΠΈ
- 2. 1. 4. 0. Π»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Ρ Π΄Π»Ρ Π°Π½Π°Π»ΠΈΠ·Π° ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΠΏΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΠΏΡΠ΅ΡΠΈΠΏΠΈΡΠ°ΡΠΈΠΈ Ρ ΡΠΎΠΌΠ°ΡΠΈΠ½Π° ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ¦Π
- 2. 1. 5. ΠΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Ρ, ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π½ΡΠ΅ Π΄Π»Ρ Π Π’-ΠΠ¦Π
- 2. 1. 6. ΠΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Ρ Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ NLS Kaiso
- 2. 2. ΠΠΎΠ»Π½ΠΎΡΠ°Π·ΠΌΠ΅ΡΠ½ΡΠ΅ ΠΊΠΠΠ Kaiso, ZBTB4, ZBTB
- 2. 3. ΠΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½Π°Ρ ΡΠ΅ΠΏΠ½Π°Ρ ΡΠ΅Π°ΠΊΡΠΈΡ
- 2. 3. Π’ΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ E. coli ΠΈ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΠΎΠΉ ΠΠΠ
- 2. 4. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ
- 2. 5. ΠΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠ¦Π -ΠΏΡΠΎΠ΄ΡΠΊΡΠ°
- 2. 6. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ°Π΄ΠΈΠΎΠ°ΠΊΡΠΈΠ²Π½ΠΎ ΠΌΠ΅ΡΠ΅Π½ΡΡ ΠΠΠ-Π·ΠΎΠ½Π΄ΠΎΠ² Π΄Π»Ρ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠΎΠ² ΠΏΠΎ ΡΠΎΡΠΌΠΎΠΆΠ΅Π½ΠΈΡ ΠΠΠ-Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² Π² Π³Π΅Π»Π΅
- 2. 7. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠΠ-Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠ³ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π·Π°Π΄Π΅ΡΠΆΠΊΠΈ ΠΏΠΎΠ΄Π²ΠΈΠΆΠ½ΠΎΡΡΠΈ Π² Π³Π΅Π»Π΅ ΠΠΠ-Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ°
- 2. 8. ΠΠΌΠΌΡΠ½ΠΎΠΏΡΠ΅ΡΠΈΠΏΠΈΡΠ°ΡΠΈΡ Ρ ΡΠΎΠΌΠ°ΡΠΈΠ½Π°
- 2. 9. ΠΠΎΠ·Π΅ΡΠ½ Π±Π»ΠΎΡ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΉ Π Π’-ΠΠ¦Π
- 2. 10. ΠΠ»Π΅ΡΠΎΡΠ½ΡΠ΅ Π»ΠΈΠ½ΠΈΠΈ ΠΈ Π²ΡΠ΅ΠΌΠ΅Π½Π½Π°Ρ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΡ
- 2. 11. ΠΠ΅ΡΠΈΠ»-Π·Π°Π²ΠΈΡΠΈΠΌΡΠΉ ΡΠ΅ΠΏΡΠ΅ΡΡΠΈΠΎΠ½ΡΠΉ ΡΠ΅ΡΡ
- 2. 12. ΠΠΌΠΌΡΠ½ΠΎΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠΈΡ ΠΈ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΡ
- 2. 13. ΠΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΈΠ½ΠΊΠΎΠ²ΡΡ ΠΏΠ°Π»ΡΡΠ΅Π² Kaiso, ZBTB4, ZBTB38 Π΄Π»Ρ in vitro ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ
- 2. 14. ΠΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠΎΠ»Π½ΠΎΡΠ°Π·ΠΌΠ΅ΡΠ½ΡΡ Kaiso, ZBTB4, ZBTB38 ΠΈ ΡΠΈΠ½ΠΊΠΎΠ²ΡΡ ΠΏΠ°Π»ΡΡΠ΅Π² Π΄Π°Π½Π½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² Π΄Π»Ρ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠΈΠΈ
- 2. 15. ΠΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π΄Π΅Π»Π΅Π½ΠΈΠΉ ZBTB4 ΠΈ ZBTB38 Π΄Π»Ρ ΠΌΠ΅ΡΠΈΠ»-Π·Π°Π²ΠΈΡΠΈΠΌΠΎΠ³ΠΎ ΡΠ΅ΠΏΡΠ΅ΡΡΠΈΠΎΠ½ΠΎΠ³ΠΎ ΡΠ΅ΡΡΠ°.56 '
- 3. 1. ΠΠ²Π° Π½ΠΎΠ²ΡΡ Π±Π΅Π»ΠΊΠ° ZBTB4 ΠΈ ZBTB38, Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΡ Kaiso, ΡΠ·Π½Π°ΡΡ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΡ ΠΠΠ in vitro
- 3. 2. ZBTB4 ΠΈ ZBTB38 ΡΠ²ΡΠ·ΡΠ²Π°ΡΡ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΡ ΠΠΠ in vivo
- 3. 3. ZBTB4 ΠΈ ZBTB38 ΡΠ²Π»ΡΡΡΡΡ ΠΌΠ΅ΡΠΈΠ»-Π·Π°Π²ΠΈΡΠΈΠΌΡΠΌΠΈ ΡΠ΅ΠΏΡΠ΅ΡΡΠΎΡΠ°ΠΌΠΈ
- 3. 4. ΠΠ°ΡΡΠΈΠ½Π° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ZBTB4 ΠΈ ZBTB
ΠΡΠ²ΠΎΠ΄Ρ.
— Π½Π°ΠΉΠ΄Π΅Π½Ρ Π΄Π²Π° Π½ΠΎΠ²ΡΡ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³Π° Kaiso: ZBTB4 ΠΈ ZBTB38, Ρ ΠΊΠΎΡΠΎΡΡΡ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½Ρ ΡΡΠΈ ΡΠΈΠ½ΠΊΠΎΠ²ΡΠ΅ ΠΏΠ°Π»ΡΡΠ° ΠΈ N-ΠΊΠΎΠ½ΡΠ΅Π²ΡΠ΅ BTB/POZ Π΄ΠΎΠΌΠ΅Π½Ρ.
— ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ZBTB4 ΠΈ ZBTB38 ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΡΡ Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠΉ ΠΠΠ in vitro, ΠΏΡΠΈΡΠ΅ΠΌ Π΄Π»Ρ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠ³ΠΎ CpGΠΊΡΠΎΠΌΠ΅ ΡΡΠΎΠ³ΠΎ ZBTB4 ΠΈΠΌΠ΅Π΅Ρ ΡΡΠΎΠ΄ΡΡΠ²ΠΎ ΠΈ ΠΊ Π½Π΅ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠΌΡ KBS ΡΠ°ΠΉΡΡ, ΡΠ°ΠΊΠΆΠ΅ ΠΊΠ°ΠΊ ΠΈ Kaiso.
— ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ZBTB4 ΠΈ ZBTB38 ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΡΡ Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠΉ ΠΠΠ in vivo. ΠΠ°Π½Π½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΠΏΡΠΈΠ²Π»Π΅ΠΊΠ°ΡΡΡΡ ΠΊ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΠΌ Ρ ΡΠΎΠΌΠΎΡΠ΅Π½ΡΡΠ°ΠΌ ΠΈ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΡΡΡ Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΠΌ DMR Π»ΠΎΠΊΡΡΠ° H19/IGF2- ΠΏΡΠΈ ΠΎΡΡΡΡΡΡΠ²ΠΈΠΈ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ dnmtl-/- Π΄Π°Π½Π½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΡΠΆΠ΅ Π½Π΅ ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΡΡ Ρ Ρ ΡΠΎΠΌΠΎΡΠ΅Π½ΡΡΠ°ΠΌΠΈ.
— ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ZBTB4 ΠΈ ZBTB38 ΡΠ²Π»ΡΡΡΡΡ ΠΌΠ΅ΡΠΈΠ»ΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΠΌΠΈ ΡΠ΅ΠΏΡΠ΅ΡΡΠΎΡΠ°ΠΌΠΈ, ΠΏΡΠΈΡΠ΅ΠΌ Π΄Π»Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅ΠΏΡΠ΅ΡΡΠΈΠΈ ZBTB4 Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΡΡΠ΅Ρ ΡΠΈΠ½ΠΊΠΎΠ²ΡΡ ΠΏΠ°Π»ΡΡΠ΅Π², Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ Π΄Π»Ρ ZBTB38 Π½Π΅ΠΎΠ±Ρ ΠΎΠ΄ΠΈΠΌΡ ΠΊΠ°ΠΊ ΡΠΈΠ½ΠΊΠΎΠ²ΡΠ΅ ΠΏΠ°Π»ΡΡΡ, ΡΠ°ΠΊ ΠΈ BTB/POZ Π΄ΠΎΠΌΠ΅Π½. ZBTB4 ΡΠ²Π»ΡΠ΅ΡΡΡ Π±ΠΎΠ»Π΅Π΅ ΡΠΈΠ»ΡΠ½ΡΠΌ ΡΠ΅ΠΏΡΠ΅ΡΡΠΎΡΠΎΠΌ, ΡΠ΅ΠΌ ZBTB38.
— ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ZBTB4 ΠΈ ZBTB38 ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΡΡΡΡ Π²ΠΎ Π²ΡΠ΅Ρ ΠΏΡΠΎΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΠΎΡΠ³Π°Π½Π°Ρ ΠΈ ΡΠΊΠ°Π½ΡΡ , Ρ ΠΎΡΡ ΠΈ Π½Π° ΡΠ°Π·Π½ΠΎΠΌ ΡΡΠΎΠ²Π½Π΅.
ΠΠ»Π°Π³ΠΎΠ΄Π°ΡΠ½ΠΎΡΡΠΈ.
Π₯ΠΎΡΡ Π²ΡΡΠ°Π·ΠΈΡΡ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ½ΠΎΡΡΡ ΡΠ²ΠΎΠ΅ΠΌΡ Π½Π°ΡΡΠ½ΠΎΠΌΡ ΡΡΠΊΠΎΠ²ΠΎΠ΄ΠΈΡΠ΅Π»Ρ ΠΡΠΎΡ ΠΎΡΡΡΠΊΡ ΠΠ³ΠΎΡΡ ΠΠΎΡΠΈΡΠΎΠ²ΠΈΡΡ Π·Π° ΡΡΡΠΊΠΎΠ΅ ΡΡΠΊΠΎΠ²ΠΎΠ΄ΡΡΠ²ΠΎ ΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΡΠ°Π±ΠΎΡΠ°ΡΡ Π½Π°Π΄ ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠ½ΡΠΌΠΈ ΡΠ΅ΠΌΠ°ΠΌΠΈ. Π’Π°ΠΊΠΆΠ΅ Ρ ΠΎΡΡ ΠΏΠΎΠ±Π»Π°Π³ΠΎΠ΄Π°ΡΠΈΡΡ ΡΠ΅Ρ , ΠΊΡΠΎ ΠΎΠΊΠ°Π·Π°Π» Π½Π΅ΠΎΡΠ΅Π½ΠΈΠΌΡΡ ΠΏΠΎΠΌΠΎΡΡ: Π‘Π°Π»ΠΎΠΆΠΈΠ½Π° Π‘Π΅ΡΠ³Π΅Ρ, ΠΡΠΎΡ ΠΎΡΡΡΠΊ ΠΠ½Π½Ρ, ΠΠΉΡΡ ΠΎΠΆΠΈΠ½Ρ ΠΠ°Π½Ρ. ΠΡΡΠ°Π·ΠΈΡΡ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ½ΠΎΡΡΡ ΠΠ°Π·ΡΡΡ ΠΠ»Π΅ΠΊΡΠ°Π½Π΄ΡΡ ΠΈ ΠΠ΅Π΅Π²Ρ ΠΠ³ΠΎΡΡ Π·Π° ΠΎΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅ ΠΈ ΠΊΡΠΈΡΠΈΠΊΡ ΡΠ°Π±ΠΎΡΡ.
ΠΡΠ΄Π΅Π»ΡΠ½ΠΎ ΠΏΠΎΠ±Π»Π°Π³ΠΎΠ΄Π°ΡΠΈΡΡ Π·Π° ΠΏΡΠ΅Π΄ΠΎΡΡΠ°Π²Π»Π΅Π½Π½ΡΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠ΅ Π»ΠΈΠ½ΠΈΠΈ ΠΏΡΠΎΡΠ΅ΡΡΠΎΡΠ° ΠΡΡΠ΄Π° ΠΈ Π·Π° ΠΏΡΠ΅Π΄ΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ ΠΌΡΡΠΈ — Π΄ΠΎΠΊΡΠΎΡΠ° ΠΠΎΠ»ΡΡΠΎΠΌ Π Π°ΠΉΠΊΠΎΠΌ, Π΄ΠΎΠΊΡΠΎΡΠ° ΠΠ΅ΡΠΎΡΡΠ΅Π·Π° Π·Π° ΠΏΡΠ΅Π΄ΠΎΡΡΠ°Π²Π»Π΅Π½Π½ΡΠ΅ Π°Π½ΡΠΈΡΠ΅Π»Π°.
Π₯ΠΎΡΡ ΠΏΠΎΠ±Π»Π°Π³ΠΎΠ΄Π°ΡΠΈΡΡ ΠΊΠ°ΡΠ΅Π΄ΡΡ Π±ΠΈΠΎΡΠΈΠ·ΠΈΠΊΠΈ Π·Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ Π²ΡΠ±ΠΈΡΠ°ΡΡ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΡ, ΠΎΡΠΎΠ±ΠΎ Ρ ΠΎΡΡ Π²ΡΡΠ°Π·ΠΈΡΡ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ½ΠΎΡΡΡ ΠΠ°ΡΠ΅Π΄Π°ΡΠ΅Π»Π΅Π²Ρ ΠΠ»Π΅ΠΊΡΠ°Π½Π΄ΡΡ Π‘Π΅ΡΠ³Π΅Π΅Π²ΠΈΡΡ.
ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅
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ΠΡΠ½ΠΎΠ²Π½ΡΠΌ Π²ΠΎΠΏΡΠΎΡΠΎΠΌ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΌΠ΅ΡΠΈΠ»-ΠΠΠ ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ², ΠΊΠ°ΠΊ ΡΠΆΠ΅ ΡΠΏΠΎΠΌΠΈΠ½Π°Π»ΠΎΡΡ Π²ΡΡΠ΅, ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΎΡ ΡΠ°ΠΊΡ, ΡΡΠΎ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠ΅, Ρ ΠΊΠΎΡΠΎΡΡΡ ΡΠ΄Π°Π»Π΅Π½Ρ Π³Π΅Π½Ρ ΠΈΠ·Π²Π΅ΡΡΠ½ΡΡ ΠΌΠ΅ΡΠΈΠ»-ΠΠΠ-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ²: ΠΠΠ2, ΠΠ΅Π‘Π 2, ΠΠ°1Π·ΠΎ, ΠΠ¬Ρ11, — Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎ ΠΏΡΠΎΡ ΠΎΠ΄ΡΡ ΡΡΠ°Π΄ΠΈΡ ΡΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ°Π·Π²ΠΈΡΠΈΡ. Ρ.
03 1— ΠΏ.
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2370 Ρ Π€ 0) ΠΎ. ΡΠΏ Π ΡΠΏ ΡΠΈ.
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Π£> 3 Π ΡΠΎ Π½ ΡΠ° N.
1350 Π.
100 Π³ ΡΠ».
00 .ΡΠΈ Ρ ΠΊ Ρ Ρ Ρ Ρ Ρ Π³.
Π¨ Ρ Ρ.
Π ΠΈΡΡΠ½ΠΎΠΊ 20. /ΠΠΠ4 ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΠ΅ΡΡΡ Π²ΠΎ Π²ΡΠ΅Ρ ΠΏΡΠΎΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΡΠΊΠ°Π½ΡΡ ΠΈ ΠΎΡΠ³Π°Π½Π°Ρ Π½Π° ΡΠ°Π·Π½ΠΎΠΌ ΡΡΠΎΠ²Π½Π΅. Π. Π½ΠΎΠ·Π΅ΡΠ½ Π±Π»ΠΎΡ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ Ρ Π ΠΠ ΠΈΠ· ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΎΡΠ³Π°Π½ΠΎΠ², Π½Π° ΠΊΠ°ΠΆΠ΄ΡΡ Π΄ΠΎΡΠΎΠΆΠΊΡ Π½Π°Π½Π΅ΡΠ΅Π½ΠΎ, Π½ΠΎ 2 ΠΌΠΊΠ³ Π ΠΠΠ²Π΅ΡΡ Π½ΡΡ ΠΏΠ°Π½Π΅Π»Ρ: Π±Π»ΠΎΡ Π±ΡΠ» Π΅Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·ΠΎΠ²Π°Π½ Ρ ΠΏΠΎΠ»Π½ΠΎΡΠ°Π·ΠΌΠ΅ΡΠ½ΠΎΠΉ ΠΊΠΠΠ Π³ΠΠ’Π4- Π½ΠΈΠΆΠ½ΡΡ ΠΏΠ°Π½Π΅Π»Ρ: Π±Π»ΠΎΡ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·ΠΎΠ²Π°Π»ΠΈ Ρ ΠΊΠΠΠ Π‘ΠΠ ΠΠ Π. Π Π’-ΠΠ¦Π Π½Π° Π ΠΠ, Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΠΎΠΉ ΠΈΠ· ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΎΡΠ³Π°Π½ΠΎΠ² ΠΈ ΡΠΌΠ±ΡΠΈΠΎΠ½ΠΎΠ². Π£ΡΠΎΠ²Π΅Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π₯ΠΠ’Π4 ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π Π’-ΠΠ¦Π ΠΈ Π½ΠΎΡΠΌΠ°Π»ΠΈΠ·ΠΎΠ²Π°Π»ΠΈ ΠΏΠΎ ΡΠΈΠ³Π½Π°Π»Ρ ΠΎΡ ΠΡΡ29 ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠ°.
Π ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΡ ΡΠΈΡΠΎΠ·ΠΈΠ½ΠΎΠ² ΠΏΠΎ ΠΏΡΡΠΎΠΌΡ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡ Π² Π³Π΅Π½ΠΎΠΌΠ΅ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π½ΠΎΠΊΠ°ΡΡΠ° Π³Π΅Π½Π° ΠΌΠ΅ΡΠΈΠ»-ΠΠΠ-ΡΡΠ°Π½ΡΡΠ΅ΡΠ°Π·Ρ dnmt-1 ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΠΌΠ΅ΡΡΠΈ ΡΠΌΠ±ΡΠΈΠΎΠ½Π° Π² ΡΠ΅ΡΠ΅Π΄ΠΈΠ½Π΅ Π³Π΅ΡΡΠ°ΡΠΈΠΈ. ΠΠ΄Π½ΠΈΠΌ ΠΈΠ· Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΡ ΠΎΠ±ΡΡΡΠ½Π΅Π½ΠΈΠΉ ΡΠ²Π»ΡΠ΅ΡΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ·Π°ΠΌΠ΅Π½ΡΠ΅ΠΌΠΎΡΡΡ Π΄Π°Π½Π½ΡΡ ΠΌΠ΅ΡΠΈΠ»-ΠΠΠ-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ². ΠΠ΄Π½Π°ΠΊΠΎ, Π½Π΅Π΄Π°Π²Π½ΠΎ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΌΡΡΡ, Π² ΠΊΠΎΡΠΎΡΠΎΠΉ ΠΏΡΡΠ΅ΠΌ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π½ΠΎΠΊΠ°ΡΡΠ° ΡΠ΄Π°Π»ΠΈΠ»ΠΈ Π³Π΅Π½Ρ ΡΡΠΈΡ ΡΡΠ΅Ρ ΠΌΠ΅ΡΠΈΠ»-ΠΠΠ-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ ΡΡ Π±Π΅Π»ΠΊΠΎΠ², ΡΠ°Π·Π²ΠΈΠ²Π°Π»Π°ΡΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ Π²ΡΠ΅Π³ΠΎ ΡΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΠ΅ΡΠΈΠΎΠ΄Π°. Π€Π΅Π½ΠΎΡΠΈΠΏ ΡΠ°ΠΊΠΎΠΉ Π²Π·ΡΠΎΡΠ»ΠΎΠΉ ΠΌΡΡΠΈ Π±ΡΠ» ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ ΡΠ°ΠΊΠΎΠΉ ΠΆΠ΅ ΠΊΠ°ΠΊ ΠΈ Ρ ΠΌΡΡΠΈ Π½ΠΎΠΊΠ°ΡΡΠ½ΠΎΠΉ ΠΏΠΎ ΠΠ΅Π‘Π 2 Π³Π΅Π½Ρ, Ρ. Π΅. Ρ ΡΠΈΠΌΠΏΡΠΎΠΌΠ°ΠΌΠΈ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΡΠΌΠΈ Π΄Π»Ρ Π Π΅ΡΡ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ°. ΠΠΎΠ»Π΅Π΅ ΡΠΎΠ³ΠΎ Π² Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ ΠΏΡΠΎΡΠ΅ΡΡΠΎΡΠ° ΠΠ΅ΡΠ΄Π° (Adrian Bird) Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ siRNA Π±ΡΠ»Π° ΠΏΠΎΠ»ΡΡΠ΅Π½Π° ΠΊΠ»Π΅ΡΠΎΡΠ½Π°Ρ Π»ΠΈΠ½ΠΈΡ ΡΠΈΠ±ΡΠΎΠ±Π»Π°ΡΡΠΎΠ² ΠΈΠ· ΠΊΠ»Π΅ΡΠΎΠΊ Ρ Π²ΠΎΡΡΠ° ΠΌΡΡΠΈ (Π½ΠΎΠΊΠ°ΡΡΠ½ΠΎΠΉ ΠΏΠΎ Mbd2, ΠΠ΅Π‘Π 2, Kaiso Π³Π΅Π½Π°ΠΌ), Π² ΠΊΠΎΡΠΎΡΠΎΠΉ ΠΎΡΡΡΡΡΡΠ²ΠΎΠ²Π°Π» Π±Π΅Π»ΠΎΠΊ Mbdl (Helle Jorgensen&Adrian Bird, personal communication). ΠΠΎΡΡΠΎΠΌΡ, Π²Π΅ΡΠΎΡΡΠ½ΠΎ, ΡΡΠΎ Π΄Π°Π½Π½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΡΠ²Π»ΡΡΡΡΡ Π½Π΅ Π΅Π΄ΠΈΠ½ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΌΠΎΠ³ΡΡ Π²ΠΎΡΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΡ ΠΠΠ.
Π Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠΈΡΠΊΠ° ΠΏΠΎ Π±Π°Π·Π΅ Π΄Π°Π½Π½ΡΡ Π΄Π°Π½Π½ΡΡ NCBI, RefSeq ΠΏΡΠΎΠ΅ΠΊΡ Π±ΡΠ»ΠΈ Π½Π°ΠΉΠ΄Π΅Π½Ρ Π΄Π²Π° Π±Π΅Π»ΠΊΠ°, Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΠ΅ Kaiso: ZBTB4 ΠΈ ZBTB38. ΠΡΠΈ Π±Π΅Π»ΠΊΠΈ ΠΈΠΌΠ΅ΡΡ ΡΡΠΈ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΡ ΡΠΈΠ½ΠΊΠΎΠ²ΡΡ ΠΏΠ°Π»ΡΡΠ° ΠΈ ΠΏΠΎΡ ΠΎΠΆΡΡ Π΄ΠΎΠΌΠ΅Π½Π½ΡΡ ΡΡΡΡΠΊΡΡΡΡ Π²ΡΠ΅Π³ΠΎ Π±Π΅Π»ΠΊΠ°: Π½Π° N-ΠΊΠΎΠ½ΡΠ΅ Π²ΡΠ΅ ΡΡΠΈ Π±Π΅Π»ΠΊΠ° ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ BTB/POZ Π΄ΠΎΠΌΠ΅Π½. ZBTB4 ΠΈ ZBTB38 Π±ΠΎΠ»Π΅Π΅ ΡΡ ΠΎΠΆΠΈ Π΄ΡΡΠ³ Ρ Π΄ΡΡΠ³ΠΎΠΌ, ΡΠ΅ΠΌ Ρ Kaiso. ΠΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ ΡΡΠΈ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π³Π΅Π½ΠΎΠ² ΡΠ²Π»ΡΡΡΡΡ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠΌ Π΄Π²ΡΡ ΡΠ°ΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΠΉ ΠΎΡ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΎΠ±ΡΠ΅Π³ΠΎ Π³Π΅Π½Π°: ΠΏΠ΅ΡΠ²ΠΎΠ΅ ΠΏΡΠΈΠ²Π΅Π»ΠΎ ΠΊ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ Kaiso-ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠ° ΠΈ ZBTB4/ZBTB38-npefliuecTBeHHHKa. Π³ΠΠ’Π4/Π³ΠΠ’Π38-ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊ Π² ΡΠ²ΠΎΡ ΠΎΡΠ΅ΡΠ΅Π΄Ρ ΠΏΡΠΈΠ²Π΅Π» ΠΊ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ZBTB4 ΠΈ ZBTB38. ΠΠ°Π½Π½Π°Ρ ΡΠ°Π±ΠΎΡΠ° ΠΏΠΎΠΊΠ°Π·Π°Π»Π°, ΡΡΠΎ Π²ΡΠ΅ ΡΡΠΈ Π±Π΅Π»ΠΊΠ° ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΡΡ Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠΉ ΠΠΠ. ΠΡΠΎΡ ΡΠ°ΠΊΡ Π³ΠΎΠ²ΠΎΡΠΈΡ Π² ΠΏΠΎΠ»ΡΠ·Ρ ΡΠΎΠ³ΠΎ, ΡΡΠΎ Π²Π΅ΡΠΎΡΡΠ½ΠΎ, ΠΎΠ±ΡΠΈΠΉ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊ Π΄Π°Π½Π½ΡΡ Π³Π΅Π½ΠΎΠ² ΡΠ°ΠΊΠΆΠ΅ Π±ΡΠ» Π½Π°Π΄Π΅Π»Π΅Π½ ΡΡΠΈΠΌ ΡΠ²ΠΎΠΉΡΡΠ²ΠΎΠΌ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΎΠ²Π°ΡΡ Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠΉ ΠΠΠ, ΠΈ ΡΠ°ΠΊΠ°Ρ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΡΠ²ΡΠ·ΡΠ²Π°ΡΡ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΡ ΠΠΠ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠΈΠ½ΠΊΠΎΠ²ΡΡ ΠΏΠ°Π»ΡΡΠ΅Π² Π΄ΠΎΠ»ΠΆΠ½Π° ΡΡΡΠ΅ΡΡΠ²ΠΎΠ²Π°ΡΡ Π² ΡΠ΅Π»ΠΎΠΌ ΡΡΠ΄Π΅ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ². ΠΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ Π²ΠΎ Π²ΡΠ΅Ρ Π²ΠΈΠ΄Π°Ρ ΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΡΡ Π±ΡΠ»ΠΈ Π½Π°ΠΉΠ΄Π΅Π½Ρ ΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈ ZBTB4 ΠΈ ZBTB38, ΡΠ°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ ΠΌΠΎΠΆΠ½ΠΎ Π²ΡΠ΄Π΅Π»ΠΈΡΡ Π΄Π²Π° ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π°: ΠΎΠ΄Π½ΠΎ ΡΠΎΠ΄Π΅ΡΠΆΠΈΡ ZBTB4, Π΄ΡΡΠ³ΠΎΠ΅ ZBTB38. Π Ρ ΠΎΡΡ Ρ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΈ Π½Π΅ Π±ΡΠ»ΠΈ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Ρ Π³Π΅Π½Ρ ΡΠΎΠ³ΠΎ ΠΈΠ»ΠΈ ΠΈΠ½ΠΎΠ³ΠΎ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π°, ΡΠΊΠΎΡΠ΅ΠΉ Π²ΡΠ΅Π³ΠΎ ΡΡΠΎ ΡΠ²ΡΠ·Π°Π½ΠΎ Ρ Π½Π΅Π·Π°ΠΊΠΎΠ½ΡΠ΅Π½Π½ΠΎΡΡΡΡ Π³Π΅Π½ΠΎΠΌΡΡ ΠΏΡΠΎΠ΅ΠΊΡΠΎΠ² Π΄Π»Ρ Π΄Π°Π½Π½ΡΡ Π²ΠΈΠ΄ΠΎΠ².
ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΡ ΠΏΠΎ Π·Π°Π΄Π΅ΡΠΆΠΊΠ΅ ΠΏΠΎΠ΄Π²ΠΈΠΆΠ½ΠΎΡΡΠΈ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² Π±Π΅Π»ΠΎΠΊ-ΠΠΠ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, ΡΡΠΎ ZBTB4, ΡΠ°ΠΊΠΆΠ΅ ΠΊΠ°ΠΊ ΠΈ Kaiso, ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ ΡΡΠΎΠ΄ΡΡΠ²ΠΎΠΌ ΠΊ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΈΠΌ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡΠΌ ΠΠΠ. In vitro, ΡΡΠΈ ΡΠΈΠ½ΠΊΠΎΠ²ΡΡ ΠΏΠ°Π»ΡΡΠ° ZBTB4, Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΠ΅ ΡΠΈΠ½ΠΊΠΎΠ²ΡΠΌ ΠΏΠ°Π»ΡΡΠ°ΠΌ Kaiso, ΠΌΠΎΠ³ΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΎΠ²Π°ΡΡ ΠΊΠ°ΠΊ Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠΉ ΠΠΠ, ΡΠ°ΠΊ ΠΈ Ρ Π½Π΅ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΠΌ KBS ΡΠ°ΠΉΡΠΎΠΌ. ΠΡΠΎΠΌΠ΅ ΡΡΠΎΠ³ΠΎ, ZBTB4 ΡΠΎΠ΄Π΅ΡΠΆΠΈΡ Π΅ΡΡ ΡΡΠΈ Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΡΡ ΡΠΈΠ½ΠΊΠΎΠ²ΡΡ ΠΏΠ°Π»ΡΡΠ° (ΡΠΈΡ.8), ΠΊΠΎΡΠΎΡΡΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ ΠΌΠΎΠ³ΡΡ ΡΡΠ°ΡΡΠ²ΠΎΠ²Π°ΡΡ Π² ΡΠ°ΡΠΏΠΎΠ·Π½Π°Π²Π°Π½ΠΈΠΈ Π΅ΡΡ ΠΊΠ°ΠΊΠΎΠΉ-Π»ΠΈΠ±ΠΎ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ. Π’Π°ΠΊΠ°Ρ ΠΊΠ°ΡΡΠΈΠ½Π° ΠΈΠΌΠ΅Π΅Ρ ΠΌΠ΅ΡΡΠΎ Π΄Π»Ρ ZBTB38 (105). Zenon ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΊΡΡΡΠΈΠ½ΡΠΌ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΎΠΌ ZBTB38. ΠΠ°ΠΊ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ Π² ΡΠ°Π±ΠΎΡΠ΅ (105) Zenon ΡΠ²ΡΠ·ΡΠ²Π°Π΅ΡΡΡ Ρ Π½Π΅ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠΉ ΠΠΠ, Π° ΡΠΎΡΠ½Π΅Π΅ Ρ Π-Π±ΠΎΠΊΡΠΎΠΌ CACCTG. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, ΠΈ ZBTB4, ΠΈ ZBTB38 ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΡΡ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠΉ ΠΠΠ, Π½ΠΎ ΠΈ Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΠΌΠΈ Π½Π΅ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΠΌΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡΠΌΠΈ.
ΠΡΠ»ΠΈ ΠΆΠ΅ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡ ΡΠΎΠ»ΡΠΊΠΎ ΡΠ²ΠΎΠΉΡΡΠ²Π° Π΄Π°Π½Π½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² ΡΠ²ΡΠ·ΡΠ²Π°ΡΡ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΡ ΠΠΠ, ΡΠΎ ZBTB4 ΠΈ ZBTB38 Π²Π΅Π΄ΡΡ ΡΠ΅Π±Ρ ΡΠ°ΠΊΠΆΠ΅ ΠΊΠ°ΠΊ ΠΈ MBD Π±Π΅Π»ΠΊΠΈ: ΡΠ²ΡΠ·ΡΠ²Π°ΡΡ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΡΠ΅ CpG in vitro, ΠΏΡΠΈΠ²Π»Π΅ΠΊΠ°ΡΡΡΡ Π½Π° Ρ ΡΠΎΠΌΠΎΡΠ΅Π½ΡΡΡ Π² ΠΌΡΡΠΈΠ½ΡΡ ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΠΈ ΠΏΠΎΠ΄Π°Π²Π»ΡΡΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ Ρ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΎΠΉ ΠΌΠ°ΡΡΠΈΡΡ.
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