ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΎΡΡΠ°ΡΠΊΠΎΠ², ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π· ΠΈ ΠΏΠ΅Π½ΠΈΡΠΈΠ»Π»ΠΈΠ½-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ², ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°
![ΠΠΈΡΡΠ΅ΡΡΠ°ΡΠΈΡ: ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ
ΠΎΡΡΠ°ΡΠΊΠΎΠ², ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΡ
ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π· ΠΈ ΠΏΠ΅Π½ΠΈΡΠΈΠ»Π»ΠΈΠ½-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ
Π±Π΅Π»ΠΊΠΎΠ², ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°](https://referat.bookap.info/work/3171786/cover.png)
ΠΠ΅ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΡΠ°ΡΡΠΈΠ΅ Π² ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ΅ ΠΈΠ»ΠΈ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° ΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎ-ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡ, ΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡΠΈΡ Π½Π΅ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΡΠ°ΡΡΠΈΠ΅ Π² ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ΅. (Π1Π°225 Π² CALB ΠΈ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈ Π² Π΄ΡΡΠ³ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°Ρ , Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Lys236 Π² ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Π°Ρ ); ΠΡΠΎΠ²Π΅Π΄Π΅Π½ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΏΠ΅Π½ΠΈΡΠΈΠ»Π»ΠΈΠ½-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ². ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ·ΠΈΡΠΈΠΈ Trp222, Trpl 14… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΎΡΡΠ°ΡΠΊΠΎΠ², ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π· ΠΈ ΠΏΠ΅Π½ΠΈΡΠΈΠ»Π»ΠΈΠ½-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ², ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- 1. Π‘ΠΏΠΈΡΠΎΠΊ ΡΠΎΠΊΡΠ°ΡΠ΅Π½ΠΈΠΉ
- 2. ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅
- 3. ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ½ΡΠΉ ΠΎΠ±Π·ΠΎΡ
- 3. 1. ΠΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ Π²ΡΡΠ°Π²Π½ΠΈΠ²Π°Π½ΠΈΠ΅ — ΠΈΡΡΠΎΡΠ½ΠΈΠΊ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΠΎ ΡΡΡΡΠΊΡΡΡΠ΅ ΠΈ ΡΡΠ½ΠΊΡΠΈΠΈ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΡ
ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²: ΠΎΡΠ½ΠΎΠ²Ρ ΠΏΠΎΡΡΡΠΎΠ΅Π½ΠΈΡ ΠΈ Π°Π½Π°Π»ΠΈΠ·Π°
- 3. 1. 1. ΠΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅
- 3. 1. 2. ΠΠ»Π³ΠΎΡΠΈΡΠΌ Π³Π»ΠΎΠ±Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΡΠ°Π²Π½ΠΈΠ²Π°Π½ΠΈΡ ΠΠΈΠ΄Π»ΠΌΠ°Π½Π°-ΠΡΠ½ΡΠ°
- 3. 1. 3. ΠΠ»Π³ΠΎΡΠΈΡΠΌ Π»ΠΎΠΊΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΡΠ°Π²Π½ΠΈΠ²Π°Π½ΠΈΡ Π‘ΠΌΠΈΡΠ°-ΠΠ°ΡΠ΅ΡΠΌΠ°Π½Π°
- 3. 1. 4. ΠΠ°ΡΡΠΈΡΡ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ Π·Π°ΠΌΠ΅Π½
- 3. 1. 5. ClustalW ΠΈ T-coffee — ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ Π°Π»Π³ΠΎΡΠΈΡΠΌΡ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π²ΡΡΠ°Π²Π½ΠΈΠ²Π°Π½ΠΈΡ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ Π±Π΅Π»ΠΊΠΎΠ² Π·Π° ΡΠ°Π·ΡΠΌΠ½ΠΎΠ΅ Π²ΡΠ΅ΠΌΡ
- 3. 1. 6. Mustang — Π°Π»Π³ΠΎΡΠΈΡΠΌ Π²ΡΡΠ°Π²Π½ΠΈΠ²Π°Π½ΠΈΡ ΡΡΠ΅Ρ ΠΌΠ΅ΡΠ½ΡΡ ΡΡΡΡΠΊΡΡΡ Π±Π΅Π»ΠΊΠΎΠ²
- 3. 2. ΠΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ²Π½ΡΠ΅ ΠΏΠΎΠ·ΠΈΡΠΈΠΈ — ΠΏΠΎΠΈΡΠΊ ΠΎΠ±ΡΠΈΡ
Π·Π°ΠΊΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠ΅ΠΉ Π² ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΊΠΈ
- 3. 2. 2. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎ-Π²Π°ΠΆΠ½ΡΡ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΎΡΡΠ°ΡΠΊΠΎΠ² ΠΏΠΎ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΠΌ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡΠΌ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π°
- 3. 2. 3. ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΡΡΠΊΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΎΡΡΠ°ΡΠΊΠΎΠ², ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ Π² ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΠΈ ΡΡΠ±ΡΡΡΠ°ΡΠ° ΠΈ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ΅
- 3. 3. ΠΠ°ΡΠΈΠ°Π±Π΅Π»ΡΠ½ΡΠ΅ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΡ, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ Π·Π° ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ Π² ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²
- 3. 3. 1. ΠΠ»Π³ΠΎΡΠΈΡΠΌΡ ΠΏΠΎΠΈΡΠΊΠ° Π²Π°ΡΠΈΠ°Π±Π΅Π»ΡΠ½ΡΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎ Π·Π½Π°ΡΠΈΠΌΡΡ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΎΡΡΠ°ΡΠΊΠΎΠ² ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΊΠΈ
- 3. 3. 2. ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ Π²Π°ΡΠΈΠ°Π±Π΅Π»ΡΠ½ΡΡ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΎΡΡΠ°ΡΠΊΠΎΠ² Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Π΅ΠΉ Π² ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°Ρ
- 3. 3. 3. ΠΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· Π²Π°ΡΠΈΠ°Π±Π΅Π»ΡΠ½ΡΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΉ Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Π΅ΠΉ Π² ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°Ρ
- 3. 4. ΠΠ±ΡΠΈΠ΅ ΡΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΎ ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·
- 3. 4. 1. ΠΡΠ½ΠΎΠ²Π½ΡΠ΅ ΡΡΡΡΠΊΡΡΡΠ½ΡΠ΅ ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·
- 3. 4. 2. ΠΠ½ΠΎΠ³ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΠ½ΠΊΡΠΈΠΈ ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·
- 3. 4. 3. ΠΠΈΠΏΠ°Π·Ρ. Π‘ΡΡΡΠΊΡΡΡΠ° ΠΈ ΡΡΠ½ΠΊΡΠΈΡ Π»ΠΈΠΏΠ°Π·Ρ Π ΠΈΠ· Candida Antarctica. Π€ΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ ΠΌΠ½ΠΎΠ³ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ Π»ΠΈΠΏΠ°Π·Ρ Π ΠΈΠ· Candida Antarctica
- 3. 4. 4. ΠΠΈΠ΄ΡΠΎΠ»Π°Π·Ρ ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΎΠ² — ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²ΠΎ ΠΊΠ°ΡΠ±ΠΎΠΊΡΠΈΠΏΠ΅ΠΏΡΠΈΠ΄Π°Π·
- 3. 4. 5. Π‘Π΅ΠΌΠ΅ΠΉΡΡΠ²ΠΎ ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·
- 3. 5. ΠΠ±ΡΠΈΠ΅ ΡΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΎ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π΅ D-Π°ΠΌΠΈΠ½ΠΎΠΏΠ΅ΠΏΡΠΈΠ΄Π°Π·
- 3. 5. 1. Π‘ΡΠ±ΡΡΡΠ°ΡΠ½Π°Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΠΈ ΠΊΠ°ΡΠ°Π»ΠΈΠ·ΠΈΡΡΠ΅ΠΌΡΠ΅ ΡΠ΅Π°ΠΊΡΠΈΠΈ
- 3. 5. 2. Π‘ΡΡΡΠΊΡΡΡΠ° ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΠΈ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΡ Π΅Π³ΠΎ Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠ΅Π½ΡΡΠ°
- 3. 5. 3. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΡΠ°ΠΉΡ-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π·Π°
- 3. 1. ΠΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ Π²ΡΡΠ°Π²Π½ΠΈΠ²Π°Π½ΠΈΠ΅ — ΠΈΡΡΠΎΡΠ½ΠΈΠΊ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΠΎ ΡΡΡΡΠΊΡΡΡΠ΅ ΠΈ ΡΡΠ½ΠΊΡΠΈΠΈ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΡ
ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²: ΠΎΡΠ½ΠΎΠ²Ρ ΠΏΠΎΡΡΡΠΎΠ΅Π½ΠΈΡ ΠΈ Π°Π½Π°Π»ΠΈΠ·Π°
- 5. 1. ΠΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΡΠ΅ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ ΡΠ²ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΡΡ ΡΠΎΠ΄ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΠΏΠΎ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΡΠΌ ΠΈ ΡΡΠ΅ΡΠΈΡΠ½ΡΠΌ ΡΡΡΡΠΊΡΡΡΠ°ΠΌ
- 5. 2. ΠΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠ° ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π²ΡΡΠ°Π²Π½ΠΈΠ²Π°Π½ΠΈΡ
- 5. 3. ΠΠΈΠ·ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΡ
- 5. 4. ΠΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠ΅ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΡΡ ΡΡΡΡΠΊΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²
- 5. 5. ΠΡΠ΅Π΄ΡΠΊΠ°Π·Π°Π½ΠΈΠ΅ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎ Π²Π°ΠΆΠ½ΡΡ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΎΡΡΠ°ΡΠΊΠΎΠ² Π² ΡΡΡΡΠΊΡΡΡΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²
- 5. 6. ΠΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ²ΠΎΠΉΡΡΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²
- 6. 1. ΠΠΎΠΈΡΠΊ Π‘ΠΠ — ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠΎΠ·ΠΈΡΠΈΠΉ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° — Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ
ΠΎΡΡΠ°ΡΠΊΠΎΠ², ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΡ
ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ Π²Π½ΡΡΡΠΈ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ², Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°
- 6. 1. 1. ΠΠΎΠ½ΡΠ΅ΠΏΡΠΈΡ ΠΈ ΡΡΡΡΠΊΡΡΡΠ° Π°Π»Π³ΠΎΡΠΈΡΠΌΠ° ΠΏΠΎΠΈΡΠΊΠ°
- 6. 1. 2. ΠΠ»Π³ΠΎΡΠΈΡΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Ρ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎ ΡΠ°Π·Π½ΡΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ
- 6. 1. 3. ΠΡΠ΅Π½ΠΊΠ° ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΠΈ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΎΡΡΠ°ΡΠΊΠΎΠ² Π² ΡΡΡΡΠΊΡΡΡΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°
- 6. 1. 4. Π Π°ΡΡΠ΅Ρ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎΡΡΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ²
- 6. 1. 5. ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ Π°Π»Π³ΠΎΡΠΈΡΠΌΠ° Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Ρ ΡΠ°Π·Π½ΡΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ
- 6. 1. 6. ΠΠ΅ΡΠΎΠ΄ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π±ΠΎΠ»ΡΡΠΈΡ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²
- 6. 2. ΠΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΏΠ΅Π½ΠΈΡΠΈΠ»Π»ΠΈΠ½-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ
Π±Π΅Π»ΠΊΠΎΠ². ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ»ΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠΎΠ·ΠΈΡΠΈΠΉ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π² ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΡΡΠ±ΡΡΡΠ°ΡΠ½ΠΎΠΉ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΠΈ Π-Π°ΠΌΠΈΠ½ΠΎΠΏΠ΅ΠΏΡΠΈΠ΄Π°Π·Ρ
- 6. 2. 1. ΠΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΏΠ΅Π½ΠΈΡΠΈΠ»Π»ΠΈΠ½-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ²
- 6. 2. 2. ΠΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠ±ΡΡΡΠ°ΡΠ½ΠΎΠΉ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΠΈ Π-Π°ΠΌΠΈΠ½ΠΎΠΏΠ΅ΠΏΡΠΈΠ΄Π°Π·Ρ ΠΈΠ· ΠΡΠΊΠ³ΠΎΡΠΎΠͺΠ°Π°Π³ΠΈΡ Π°Π«Π³ΠΎΡΠ³ ΠΊ Π-ΡΠ΅Π½ΠΈΠ»Π°Π»Π°Π½ΠΈΠ½Π°ΠΌΠΈΠ΄Ρ ΠΏΡΡΠ΅ΠΌ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π‘ΠΠ
- 6. 3. ΠΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π· Π½Π° ΡΡΠΎΠ²Π½Π΅ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΡΡΡΡΠΊΡΡΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠ΅Π½ΡΡΠΎΠ²
- 6. 3. 1. ΠΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π· Ρ Π»ΠΈΠΏΠ°Π·Π½ΠΎΠΉ, ΠΏΠ΅ΠΏΡΠΈΠ΄Π°Π·Π½ΠΎΠΉ ΠΈ ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΠΌΠΈ
- 6. 3. 2. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ»ΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΉ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π² ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΠΈ Π»ΠΈΠΏΠ°Π·Π½ΠΎΠΉ ΠΈ Π°ΠΌΠΈΠ΄Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠ΅ΠΉ Π² Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·Π°Ρ
- 6. 3. 3. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ»ΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΉ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π² ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΠΈ ΡΡΡΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΠΈ ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠ΅ΠΉ Π² Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·Π°Ρ
- 6. 3. 4. Π ΠΎΠ»Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΉ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π² ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠ΅ΠΉ Π² Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·Π°Ρ
8. ΠΡΠ½ΠΎΠ²Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈ Π²ΡΠ²ΠΎΠ΄Ρ.
1. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ Π°Π»Π³ΠΎΡΠΈΡΠΌ ΠΏΠΎΠΈΡΠΊΠ° ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΉ Π² ΡΡΡΡΠΊΡΡΡΠ΅ Π±Π΅Π»ΠΊΠ° (ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΉ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π°) Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π°Π½Π°Π»ΠΈΠ·Π° Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΈ ΡΡΡΡΠΊΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈΠ°Π»Π³ΠΎΡΠΈΡΠΌ, ΡΠΏΠΎΡΠΎΠ±Π½ΡΠΉ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°ΡΡ Π½Π° Π²ΡΠ΅Ρ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΡ ΡΠΈΡΡΠ΅ΠΌΠ°Ρ , ΡΠ΅Π°Π»ΠΈΠ·ΠΎΠ²Π°Π½ Π² Π²ΠΈΠ΄Π΅ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠ΄Π° Π΄Π»Ρ ΠΠΠ ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ Π² ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ΅ Π΄Π»Ρ ΡΠ°Π±ΠΎΡΡ Π² ΠΏΠ°ΡΠ°Π»Π»Π΅Π»ΡΠ½ΠΎΠΌ ΡΠ΅ΠΆΠΈΠΌΠ΅ Π½Π° ΡΡΠΏΠ΅ΡΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ΅;
2. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΌΠ΅ΡΠΎΠ΄ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π±ΠΎΠ»ΡΡΠΈΡ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Π½Π° ΡΡΠΎΠ²Π½Π΅ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ, ΠΏΠΎΠ»Π½ΠΎΡΠ°Π·ΠΌΠ΅ΡΠ½ΡΡ ΡΡΡΡΠΊΡΡΡ ΠΈ ΡΡΠΎΠ²Π½Π΅ ΡΡΡΡΠΊΡΡΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ ΡΠ΅Π½ΡΡΠΎΠ²;
3. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠ΄Π°Π»Π΅Π½Π½ΡΡ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΎΠ² ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π· Ρ Π»ΠΈΠΏΠ°Π·Π½ΠΎΠΉ, Π°ΠΌΠΈΠ΄Π°Π·Π½ΠΎΠΉ ΠΈ ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΠΌΠΈ. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ ΡΠΏΠΈΡΠΎΠΊ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΡΡ ΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ²Π½ΡΡ ΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΉ;
4. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ·ΠΈΡΠΈΠΈ ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ Π·Π° Π΄ΠΈΡΠΊΡΠΈΠΌΠΈΠ½Π°ΡΠΈΡ Π»ΠΈΠΏΠ°Π·Π½ΠΎΠΉ ΠΈ Π°ΠΌΠΈΠ΄Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠ΅ΠΉ (Π’Π³Ρ104, Ala225, Aspl34, Glnl57 ΠΈ Leu278 Π² Π»ΠΈΠΏΠ°Π·Π΅ Π ΠΈΠ· Candida antarctica);
5. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ·ΠΈΡΠΈΠΈ ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ Π·Π° Π΄ΠΈΡΠΊΡΠΈΠΌΠΈΠ½Π°ΡΠΈΡ ΡΡΡΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΠΈ ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠ΅ΠΉ (Thrl 1, Hisl4 ΠΈ Lys236 Π² ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Π΅ ΠΈΠ· Hevea brasiliensis);
6. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΏΠ΅Π½ΠΈΡΠΈΠ»Π»ΠΈΠ½-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ². ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ·ΠΈΡΠΈΠΈ Trp222, Trpl 14 ΠΈ 11Π΅220, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ Π·Π° ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΠΈ D-Π°ΠΌΠΈΠ½ΠΎΠΏΠ΅ΠΏΡΠΈΠ΄Π°Π·Ρ ΠΈΠ· Ochrobactrum anthropi ΠΊ Π°ΡΠΈΠ»ΡΠ½ΠΎΠΉ ΡΠ°ΡΡΠΈ ΡΡΠ±ΡΡΡΠ°ΡΠ°;
7. ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Π° ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½Π°Ρ ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΉ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π² ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π°Ρ Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π· ΠΈ ΠΏΠ΅Π½ΠΈΡΠΈΠ»Π»ΠΈΠ½ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠ°Ρ .
8. ΠΠ»Π°Π³ΠΎΠ΄Π°ΡΠ½ΠΎΡΡΠΈ.
ΠΠ²ΡΠΎΡ Π²ΡΡΠ°ΠΆΠ°Π΅Ρ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ½ΠΎΡΡΡ ΡΠΎΡΡΡΠ΄Π½ΠΈΠΊΠ°ΠΌ ΠΊΠΎΠΌΠΏΠ°Π½ΠΈΠΈ Novozymes A/S Allan Svendsen ΠΈ Werner Besenmatter Π·Π° ΠΏΡΠ΅Π΄ΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π°ΠΌΠΈΠ΄Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π² ΠΏΡΠ΅Π΄ΡΠΊΠ°Π·Π°Π½Π½ΡΡ Π½Π°ΠΌΠΈ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ Π²Π°ΡΠΈΠ°ΡΠΈΡΡ Π»ΠΈΠΏΠ°Π·Ρ Π ΠΈΠ· Candida antarctica, ΡΠΎΡΡΡΠ΄Π½ΠΈΠΊΠ°ΠΌ Π’Π΅Ρ Π½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ½ΠΈΠ²Π΅ΡΡΠΈΡΠ΅ΡΠ° Π³. ΠΠ΅Π»ΡΡΡΠ° Jianfeng Jin, Linda Otten ΠΈ Ulf Hanefeld Π·Π° ΠΏΡΠ΅Π΄ΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΡΡΠ΅ΡΠ°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π² ΠΏΡΠ΅Π΄ΡΠΊΠ°Π·Π°Π½Π½ΡΡ Π½Π°ΠΌΠΈ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ Π²Π°ΡΠΈΠ°ΡΠΈΡΡ ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Ρ ΠΈΠ· Manihot esculenta, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠΎΡ. Π. Π. ΠΠΈΡΠΎΠ½ΠΎΠ²Ρ ΠΈ Π. Π‘. ΠΠ΅Π»ΡΡΠ°Π½Π΄Ρ Π·Π° ΠΏΠ»ΠΎΠ΄ΠΎΡΠ²ΠΎΡΠ½ΡΠ΅ Π±Π΅ΡΠ΅Π΄Ρ ΠΈ ΠΎΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΡ.
7.
ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅
.
Π ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠ²ΠΎΠ»ΡΡΠΈΠΈ Π±Π΅Π»ΠΊΠΈ ΡΠ°Π·Π²ΠΈΠ²Π°ΡΡΡΡ ΠΎΡ ΠΎΠ±ΡΠ΅Π³ΠΎ ΠΏΡΠ΅Π΄ΠΊΠ° ΠΏΡΡΠ΅ΠΌ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ' ΡΡΡΡΠΊΡΡΡΡ — Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ Π·Π°ΠΌΠ΅Π½, Π²ΡΡΠ°Π²ΠΎΠΊ ΠΈ Π΄Π΅Π»Π΅ΡΠΈΠΉ — ΠΈ, ΡΠ°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, Π΄Π°ΡΡ Π½Π°ΡΠ°Π»ΠΎ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΌΡ ΠΌΠ½ΠΎΠ³ΠΎΠΎΠ±ΡΠ°Π·ΠΈΡ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ². ΠΠΎΠ·ΠΈΡΠΈΠΈ Π² ΡΡΡΡΠΊΡΡΡΠ΅ Π±Π΅Π»ΠΊΠΎΠ² ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΡΠ°Π·Π½ΠΎΠΉ Π²Π°ΡΠΈΠ°Π±Π΅Π»ΡΠ½ΠΎΡΡΡΡ Π² Ρ ΠΎΠ΄Π΅ Π΅ΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΎΡΠ±ΠΎΡΠ° — Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΡ Π²Π°ΠΆΠ½Ρ Π΄Π»Ρ ΡΡΠ½ΠΊΡΠΈΠΈ, ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΠΈ ΠΈΠ»ΠΈ ΡΡΡΡΠΊΡΡΡΡ ΠΈ ΠΏΠΎΡΡΠΎΠΌΡ ΡΠ²ΡΠ·Π°Π½Ρ ΠΎΡΠΎΠ±ΡΠΌΠΈ ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½ΠΈΡΠΌΠΈ Π½Π° ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΠΎΡΡΡ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠ΅ ΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΡΡΠΊΡΡΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠ΅ΠΉ Π±Π΅Π»ΠΊΠΎΠ² ΠΈ ΠΈΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΠΎΠ΄Π½Ρ ΠΈΠ· ΡΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ Π±ΠΈΠΎΡ ΠΈΠΌΠΈΠΈ.
ΠΡΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΠΈ Π΄ΠΈΡΡΠ΅ΡΡΠ°ΡΠΈΠΈ Π±ΡΠ» ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΌΠ΅ΡΠΎΠ΄ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΡΠ²ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΠΎ ΡΠ΄Π°Π»Π΅Π½Π½ΡΡ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΎΠ² Π½Π° ΡΡΠΎΠ²Π½Π΅ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π²Π°ΠΆΠ½ΡΡ Ρ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΡΠΎΡΠΊΠΈ Π·ΡΠ΅Π½ΠΈΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΡΡΡΡΠΊΡΡΡΡ — Π°ΠΊΡΠΈΠ²Π½ΡΡ ΡΠ΅Π½ΡΡΠΎΠ², Ρ. Π΅. Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΎΡΡΠ°ΡΠΊΠΎΠ², ΡΠΎΡΠΌΠΈΡΡΡΡΠΈΡ ΡΡΠ°ΡΡΠΊΠΈ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ ΡΡΠ±ΡΡΡΠ°ΡΠ° ΠΈ ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ Π² ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ΅ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠ΅Π²ΡΠ°ΡΠ΅Π½ΠΈΡ. ΠΡΠΎ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΈΠ·ΡΡΠ°ΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΡ, ΠΏΡΠΎΠΈΠ·ΠΎΡΠ΅Π΄ΡΠΈΠ΅ ΠΎΡ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΏΡΠ΅Π΄ΠΊΠ° Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΡΠ²ΠΎΠ»ΡΡΠΈΠΈ, Π½ΠΎ ΠΏΡΠ΅ΡΠ΅ΡΠΏΠ΅Π²ΡΠΈΠ΅ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² Ρ ΠΎΠ΄Π΅ Π΅ΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΎΡΠ±ΠΎΡΠ° ΠΈ, ΡΠ°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΎΡ Π²Π°ΡΠΈΡΡ Π±ΠΎΠ»ΡΡΠΎΠ΅ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ². ΠΡΠ»ΠΈ ΠΈΠ·ΡΡΠ΅Π½Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ·ΠΈΡΠΈΠΈ Π² ΡΡΡΡΠΊΡΡΡΠ°Ρ Π±Π΅Π»ΠΊΠΎΠ² ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π°, ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΠΎΡΡΡ ΠΊΠΎΡΠΎΡΡΡ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠ²ΠΎΠ»ΡΡΠΈΠΈ ΠΏΡΠΈΠ²Π΅Π»Π° ΠΊ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΡ ΠΈΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ. ΠΡΠ» ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΈ ΡΠ΅Π°Π»ΠΈΠ·ΠΎΠ²Π°Π½ Π² Π²ΠΈΠ΄Π΅ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠ΄Π° Π΄Π»Ρ ΠΠΠ Π°Π»Π³ΠΎΡΠΈΡΠΌ ΠΏΠΎΠΈΡΠΊΠ° ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΉ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π°Π½Π°Π»ΠΈΠ·Π° Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΈ ΡΡΡΡΠΊΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΌΠ΅ΡΠΎΠ΄ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π±ΠΎΠ»ΡΡΠΈΡ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ². ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Π½ΡΠΉ ΠΏΠΎΠ΄Ρ ΠΎΠ΄ Π±ΡΠ» ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ Π΄Π»Ρ Π°Π½Π°Π»ΠΈΠ·Π° ΡΠ΄Π°Π»Π΅Π½Π½ΡΡ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΎΠ² ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π· Ρ Π»ΠΈΠΏΠ°Π·Π½ΠΎΠΉ, Π°ΠΌΠΈΠ΄Π°Π·Π½ΠΎΠΉ ΠΈ ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΠΌΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΏΠ΅Π½ΠΈΡΠΈΠ»Π»ΠΈΠ½-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ². ΠΡΠ»ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ·ΠΈΡΠΈΠΈ ΡΡΠΏΠ΅ΡΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Π°Π»ΡΡΠ°-Π±Π΅ΡΠ° Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ Π·Π° Π΄ΠΈΡΠΊΡΠΈΠΌΠΈΠ½Π°ΡΠΈΡ Π»ΠΈΠΏΠ°Π·Π½ΠΎΠΉ ΠΈ Π°ΠΌΠΈΠ΄Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠ΅ΠΉ (Π’Π³Ρ104, Π1Π°225, Aspl34, Glnl57 ΠΈ Leu278 Π² Π»ΠΈΠΏΠ°Π·Π΅ Π ΠΈΠ· Candida antarctica), Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΡΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΠΈ ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠ΅ΠΉ (Thrll, His 14 ΠΈ Lys236 Π² ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Π΅ ΠΈΠ· Hevea brasiliensis). ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΠΏΠΎΠ·ΠΈΡΠΈΠΈ, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ Π·Π° ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΠΈ D-Π°ΠΌΠΈΠ½ΠΎΠΏΠ΅ΠΏΡΠΈΠ΄Π°Π·Ρ ΠΈΠ· Ochrobactrum anthropi ΠΊ Π°ΡΠΈΠ»ΡΠ½ΠΎΠΉ ΡΠ°ΡΡΠΈ ΡΡΠ±ΡΡΡΠ°ΡΠ° (222W, 114W ΠΈ 2201).
Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ Π°Π½Π°Π»ΠΈΠ·Π° ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΠΊΠ»Π°ΡΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°ΡΡ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΠ΅ ΠΎΡΡΠ°ΡΠΊΠΈ Π² ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΡΡ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΏΠΎ ΡΠ»Π΅Π΄ΡΡΡΠΈΠΌ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΌ ΡΠΈΠΏΠ°ΠΌ:
1. ΠΠ΅ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΡΠ°ΡΡΠΈΠ΅ Π² ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ΅ ΠΈΠ»ΠΈ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° ΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎ-ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡ, ΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡΠΈΡ Π½Π΅ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΡΠ°ΡΡΠΈΠ΅ Π² ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ΅. (Π1Π°225 Π² CALB ΠΈ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈ Π² Π΄ΡΡΠ³ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°Ρ , Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Lys236 Π² ΠΎΠΊΡΠΈΠ½ΠΈΡΡΠΈΠ»Π°Π·Π°Ρ );
2. Π‘ΡΠ°Π±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΡ ΠΏΠ΅ΡΠ΅Ρ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΈΠ»ΠΈ ΠΈΠ½ΡΠ΅ΡΠΌΠ΅Π΄ΠΈΠ°ΡΠ° (Thr40 ΠΈ GlnlOo Π² CALB ΠΈ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈ Π² Π΄ΡΡΠ³ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°Ρ ).
3. Π£ΡΠ°ΡΡΠΈΠ΅ Π² ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΠΈ ΡΡΠ±ΡΡΡΠ°ΡΠ° (Gly39 ΠΈ Π’Π³Ρ104 Π² CALB ΠΈ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈ Π² Π΄ΡΡΠ³ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°Ρ , Π° ΡΠ°ΠΊΠΆΠ΅ Π’Π³Ρ222, Π’Π³Ρ114 ΠΈ 11Π΅220, Π² D-Π°ΠΌΠΈΠ½ΠΎΠΏΠ΅ΠΏΡΠΈΠ΄Π°Π·Π΅ ΠΈΠ· Ochrobactrum anthropi).
4. Π‘ΡΡΡΠΊΡΡΡΠ½Π°Ρ ΡΠΎΠ»Ρ — ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅ΡΠΈ ΡΡΠ°Π±ΠΈΠ»ΠΈΠ·ΠΈΡΡΡΡΠΈΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ.
Thr40, GlnlOo, Asn79 ΠΈ Met72 Π² CALB ΠΈ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈ Π² Π΄ΡΡΠ³ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°Ρ ).
ΠΠΎΠΆΠ½ΠΎ ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠΈΡΡ ΡΡΠ°ΡΡΠΈΠ΅ Π‘ΠΠ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ , Π½Π΅ ΠΎΡΠ½ΠΎΡΡΡΠΈΡ ΡΡ Π½Π°ΠΏΡΡΠΌΡΡ ΠΊ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Ρ — ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π°ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΊΠΎΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΡΡΡΡΠΊΡΡΡΡ Π±Π΅Π»ΠΊΠ° Π·Π° ΡΡΠ΅Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ°Π±ΠΈΠ»ΠΈΠ·ΠΈΡΡΡΡΠΈΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ, ΡΡΠ°ΡΡΠΈΠ΅ Π²ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΈ Π±Π΅Π»ΠΊΠ° Ρ Π²Π½Π΅ΡΠ½ΠΈΠΌΠΈ ΠΎΠ±ΡΠ΅ΠΊΡΠ°ΠΌΠΈ, Π½Π°ΠΏΡΠΈΠΌΠ΅Ρ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½ΠΎΠΉ, ΠΈ Ρ. Π΄.
ΠΡΠΎΠ³ΡΠ°ΠΌΠΌΠ° ΠΏΠΎΠΈΡΠΊΠ° ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΉ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² ΠΈ ΠΌΠ΅ΡΠΎΠ΄ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΠΏΡΠΈ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠΈ ΡΠ²ΠΎΠ»ΡΡΠΈΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ², ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΡΡΡΡΠΊΡΡΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ ΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΡΡ ΡΠ΅Π½ΡΡΠΎΠ² ΠΈ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ° Π΄Π΅ΠΉΡΡΠ²ΠΈΡ. Π‘ΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΡΠ΄Π°Π»Π΅Π½Π½ΡΡ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΎΠ² ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΈΠ·ΡΡΠ°ΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΊΠ»Π°ΡΡΠΎΠ² ΠΈ ΠΎΡ Π²Π°ΡΠΈΡΡ ΡΠΈΡΠΎΠΊΠΎΠ΅ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ². ΠΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡ ΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΡΡ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π° Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Ρ Π·Π°Π΄Π°Π½Π½ΡΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ ΠΈ ΠΎΡΠΊΡΡΠ²Π°Π΅Ρ ΠΏΡΠΈΠ½ΡΠΈΠΏΠΈΠ°Π»ΡΠ½ΠΎ Π½ΠΎΠ²ΡΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ Π΄ΠΈΠ·Π°ΠΉΠ½Π° Π±ΠΈΠΎΠΊΠ°ΡΠ°Π»ΠΈΠ·Π°ΡΠΎΡΠΎΠ². J.
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