ΠŸΠΎΠΌΠΎΡ‰ΡŒ Π² написании студСнчСских Ρ€Π°Π±ΠΎΡ‚
АнтистрСссовый сСрвис

Π”ΠΈΠ½Π°ΠΌΠΈΠΊΠ° формирования ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… Π°Π΄Π³Π΅Π·ΠΈΠΎΠ½Π½Ρ‹Ρ… ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚ΠΎΠ² ΠΈ пСрСстроСк Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта нСтрансформированных ΠΈ трансформированных ΠΊΠ»Π΅Ρ‚ΠΎΠΊ

Π”ΠΈΡΡΠ΅Ρ€Ρ‚Π°Ρ†ΠΈΡΠŸΠΎΠΌΠΎΡ‰ΡŒ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈΠ£Π·Π½Π°Ρ‚ΡŒ ΡΡ‚ΠΎΠΈΠΌΠΎΡΡ‚ΡŒΠΌΠΎΠ΅ΠΉ Ρ€Π°Π±ΠΎΡ‚Ρ‹

Π₯ΠΎΡ€ΠΎΡˆΠΎ извСстны различия Π² ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта ΠΈ ΠΠš ΠΊΠ»Π΅Ρ‚ΠΎΠΊ Π΄Π²ΡƒΡ… Ρ‚ΠΊΠ°Π½Π΅Π²Ρ‹Ρ… Ρ‚ΠΈΠΏΠΎΠ²: эпитСлиоцитов ΠΈ Ρ„ибробластов. АК ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠΎΡ†ΠΈΡ‚ΠΎΠ² ΠΎΠ±Ρ€Π°Π·ΠΎΠ²Π°Π½Ρ‹ Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½ΠΎΠΌ, ΠΈΠΌΠ΅ΡŽΡ‚ Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½ΡƒΡŽ ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΡŽ ΠΈ ΡΠ²ΡΠ·Π°Π½Ρ‹ с «ΠΏΠ΅Ρ€ΠΈΡ„СричСским Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²Ρ‹ΠΌ ΠΏΡƒΡ‡ΠΊΠΎΠΌ. ΠΠΎΡ€ΠΌΠ°Π»ΡŒΠ½Ρ‹Π΅ фибробласты ΠΈΠΌΠ΅ΡŽΡ‚ Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½ΡƒΡŽ ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΡŽ АК, ΠΎΡ€ΠΈΠ΅Π½Ρ‚ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… пСрпСндикулярно ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π³Ρ€Π°Π½ΠΈΡ†Π΅ ΠΈ Π°ΡΡΠΎΡ†ΠΈΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… с ΠΏΡ€ΡΠΌΡ‹ΠΌΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²Ρ‹ΠΌΠΈ… Π§ΠΈΡ‚Π°Ρ‚ΡŒ Π΅Ρ‰Ρ‘ >

Π”ΠΈΠ½Π°ΠΌΠΈΠΊΠ° формирования ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… Π°Π΄Π³Π΅Π·ΠΈΠΎΠ½Π½Ρ‹Ρ… ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚ΠΎΠ² ΠΈ пСрСстроСк Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта нСтрансформированных ΠΈ трансформированных ΠΊΠ»Π΅Ρ‚ΠΎΠΊ (Ρ€Π΅Ρ„Π΅Ρ€Π°Ρ‚, курсовая, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½Ρ‚Ρ€ΠΎΠ»ΡŒΠ½Π°Ρ)

Π‘ΠΎΠ΄Π΅Ρ€ΠΆΠ°Π½ΠΈΠ΅

  • 1. Бписок сокращСний
  • 2. Π’Π²Π΅Π΄Π΅Π½ΠΈΠ΅
    • 2. 1. ΠΠΊΡ‚ΡƒΠ°Π»ΡŒΠ½ΠΎΡΡ‚ΡŒ ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌΡ‹
    • 2. 2. Π¦Π΅Π»ΠΈ ΠΈ Π·Π°Π΄Π°Ρ‡ΠΈ Ρ€Π°Π±ΠΎΡ‚Ρ‹
    • 2. 3. Научная Π½ΠΎΠ²ΠΈΠ·Π½Π° ΠΈ ΠΏΡ€Π°ΠΊΡ‚ичСская Π·Π½Π°Ρ‡ΠΈΠΌΠΎΡΡ‚ΡŒ Ρ€Π°Π±ΠΎΡ‚Ρ‹
  • 3. ΠžΠ±Π·ΠΎΡ€ Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Ρ‹
    • 3. 1. ΠšΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½Ρ‹ ΠΈ ΠΈΡ… Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΈ Π² Π½ΠΎΡ€ΠΌΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΈ Ρ‚рансформированных ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ…
      • 3. 1. 1. ΠšΠ»Π°ΡΡΠΈΡ‡Π΅ΡΠΊΠΈΠ΅ ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½Ρ‹. И
      • 3. 1. 2. ΠšΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½Ρ‹ Π΄Ρ€ΡƒΠ³ΠΈΡ… Ρ‚ΠΈΠΏΠΎΠ²
      • 3. 1. 3. ΠœΠΎΠ»Π΅ΠΊΡƒΠ»ΡΡ€Π½Π°Ρ организация ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½-содСрТащих АК
      • 3. 1. 4. ΠŸΡ€ΠΎΡ†Π΅ΡΡ сборки АК
      • 3. 1. 5. ΠΠ°Ρ€ΡƒΡˆΠ΅Π½ΠΈΡ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π°Π΄Π³Π΅Π·ΠΈΠΈ ΠΏΡ€ΠΈ нСопластичСской трансформации
    • 3. 2. Актиновый цитоскСлСт Π½ΠΎΡ€ΠΌΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΈ Ρ‚рансформированных ΠΊΠ»Π΅Ρ‚ΠΎΠΊ
      • 3. 2. 1. Актиновая ΡΠ΅Ρ‚ΡŒ ΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠ³ΠΎ края
      • 3. 2. 2. Актин-ΡΠ²ΡΠ·Ρ‹Π²Π°ΡŽΡ‰ΠΈΠ΅ Π±Π΅Π»ΠΊΠΈ
      • 3. 2. 3. БтрСсс-Ρ„ΠΈΠ±Ρ€ΠΈΠ»Π»Ρ‹
      • 3. 2. 4. ΠžΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΡ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта фибробластов
      • 3. 2. 5. ΠžΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΡ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ…
      • 3. 2. 6. УчастиС Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта Π² ΠΏΠΎΡΡ‚Ρ€ΠΎΠ΅Π½ΠΈΠΈ АК
      • 3. 2. 7. ИзмСнСния Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта ΠΏΡ€ΠΈ нСопластичСской трансформации
    • 3. 3. Π’ΠΊΠ»Π°Π΄ ΠΌΠ°Π»Ρ‹Ρ… Π“Π’Π€Π°Π· сСмСйства Rho Π² Ρ€Π΅Π³ΡƒΠ»ΡΡ†ΠΈΡŽ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта ΠΈ ΡΠ±ΠΎΡ€ΠΊΠΈ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… Π°Π΄Π³Π΅Π·ΠΈΠΎΠ½Π½Ρ‹Ρ… ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚ΠΎΠ²
      • 3. 3. 1. ΠœΠ°Π»Ρ‹Π΅ Π“Π’Π€Π°Π·Ρ‹ сСмСйства Rho
      • 3. 3. 2. Rae
      • 3. 3. 3. Rho
      • 3. 3. 4. Роль ΠΌΠ°Π»Ρ‹Ρ… Π“Π’Π€Π°Π· Rho ΠΈ Rae Π² Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ АК
      • 3. 3. 5. ИзмСнСниС рСгуляторной Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΈ ΠΌΠ°Π»Ρ‹Ρ… Π“Π’Π€Π°Π· сСмСйства Rho ΠΏΡ€ΠΈ нСопластичСской трансформации
  • 4. ΠœΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Ρ‹ ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Ρ‹
    • 4. 1. Π Π΅Π°Π³Π΅Π½Ρ‚Ρ‹
    • 4. 2. ΠšΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Π΅ Π»ΠΈΠ½ΠΈΠΈ ΠΈ Ρ‚рансфСкция
    • 4. 3. Анализ формирования ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚ΠΎΠ² Π² ΡƒΠ·ΠΊΠΎΠΉ Ρ€Π°Π½Π΅
    • 4. 4. Π’Ρ‹Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΌΠ°Π»Ρ‹Ρ… Π“Π’Π€Π°Π· Rho ΠΈ Rae ΠΈ ΠΈΡ… Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ Π² ΠΊΠ»Π΅Ρ‚ΠΊΠΈ
    • 4. 5. РНК интСрфСрСнция
    • 4. 6. ЀлуорСсцСнтноС ΠΎΠΊΡ€Π°ΡˆΠΈΠ²Π°Π½ΠΈΠ΅ ΠΈ ΠΌΠΈΠΊΡ€ΠΎΡΠΊΠΎΠΏΠΈΡ
    • 4. 7. Π’ΠΈΠ΄Π΅ΠΎΡΡŒΡ‘ΠΌΠΊΠ° ΠΈ Π°Π½Π°Π»ΠΈΠ· Π²ΠΈΠ΄Π΅ΠΎΠΈΠ·ΠΎΠ±Ρ€Π°ΠΆΠ΅Π½ΠΈΠΉ
    • 4. 8. ВСстСрн-Π±Π»ΠΎΡ‚Ρ‚ΠΈΠ½Π³
  • 5. Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹
    • 5. 1. ΠžΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΡ ΠΠš ΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСтатрансформированных эпитСлиоцитов IAR-2 ΠΈ Ρ‚рансформированных эпитСлиоцитов IAR
    • 5. 2. Анализ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½-содСрТащих АК
      • 5. 2. 1. Анализ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ ΠΠš Π² Ρ€Π΅Π΄ΠΊΠΎΠΉ ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π΅
      • 5. 2. 2. Π”ΠΈΠ½Π°ΠΌΠΈΠΊΠ° формирования ΠΠš ΠΏΡ€ΠΈ схоТдСнии ΡƒΠ·ΠΊΠΎΠΉ Ρ€Π°Π½Ρ‹
      • 5. 2. 3. ИсслСдованиС аккумуляции Π°ΠΊΡ‚ΠΈΠ½Π° ΠΈ ΠΏΡ€ΠΈ Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ АК
      • 5. 2. 4. ИсслСдованиС аккумуляции зиксина ΠΏΡ€ΠΈ Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ АК
      • 5. 2. 5. ИсслСдованиС аккумуляции Π°-Π°ΠΊΡ‚ΠΈΠ½ΠΈΠ½Π° ΠΏΡ€ΠΈ Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ ΠΠš трансформированных эпитСлиоцитов IAR
    • 5. 3. РСгуляция формирования ΠΠšΡ‚рансформированных ΠΈ Ρ‚рансформированных эпитСлиоцитов ΠΌΠ°Π»Ρ‹ΠΌΠΈ Π“Π’Π€Π°Π·Π°ΠΌΠΈ сСмСйства Rho
      • 5. 3. 1. ВлияниС ввСдСния Π‘Π— трансфСразы Π½Π° Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹Ρ… Ρ‚ΠΈΠΏΠΎΠ² АК
      • 5. 3. 2. ВлияниС ΠΈΠ½Π³ΠΈΠ±ΠΈΡ‚ΠΎΡ€Π° ROCK V-27 632 Π½Π° ΡΠ±ΠΎΡ€ΠΊΡƒ АК
      • 5. 3. 3. ДСйствиС ΠΈΠ½Π³ΠΈΠ±ΠΈΡ‚ΠΎΡ€Π° АВЀазы ΠΌΠΈΠΎΠ·ΠΈΠ½Π° блСббистатина Π½Π° ΡΠ±ΠΎΡ€ΠΊΡƒ
      • 5. 3. 4. ΠšΠΎΠ»ΠΈΡ‡Π΅ΡΡ‚Π²Π΅Π½Π½Π°Ρ ΠΎΡ†Π΅Π½ΠΊΠ° влияния Y-27 632 ΠΈ Π±Π»Π΅Π±Π±ΠΈΡΡ‚Π°Ρ‚ΠΈΠ½Π° Π½Π° Π°ΠΊΠΊΡƒΠΌΡƒΠ»ΡΡ†ΠΈΡŽ ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½Π° Π² ΠΠš Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹Ρ… Ρ‚ΠΈΠΏΠΎΠ² ΠΊΠ»Π΅Ρ‚ΠΎΠΊ
      • 5. 3. 5. ВлияниС подавлСния экспрСссии mDial Π½Π° Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ АК
      • 5. 3. 6. ВлияниС ввСдСния N17Rac Π½Π° Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ АК
  • 6. ΠžΠ±ΡΡƒΠΆΠ΄Π΅Π½ΠΈΠ΅

2.1. ΠΠΊΡ‚ΡƒΠ°Π»ΡŒΠ½ΠΎΡΡ‚ΡŒ ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌΡ‹.

ΠœΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Π΅ Π°Π΄Π³Π΅Π·ΠΈΠΎΠ½Π½Ρ‹Π΅ ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚Ρ‹ (АК) ΠΎΠ±Ρ€Π°Π·ΠΎΠ²Π°Π½Ρ‹ ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½Π°ΠΌΠΈ, ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ Ρ‡Π΅Ρ€Π΅Π· слоТный Π±Π΅Π»ΠΊΠΎΠ²Ρ‹ΠΉ комплСкс Π°Π΄Π³Π΅Π·ΠΈΠΎΠ½Π½ΠΎΠΉ бляшки ассоциированы с Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²Ρ‹ΠΌΠΈ ΠΌΠΈΠΊΡ€ΠΎΡ„ΠΈΠ»Π°ΠΌΠ΅Π½Ρ‚Π°ΠΌΠΈ. АК Π½ΠΎΡ€ΠΌΠ°Π»ΡŒΠ½Ρ‹Ρ… ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΠΈΠ³Ρ€Π°ΡŽΡ‚ Π²Π°ΠΆΠ½Π΅ΠΉΡˆΡƒΡŽ Ρ€ΠΎΠ»ΡŒ Π² ΠΏΠΎΠ΄Π΄Π΅Ρ€ΠΆΠ°Π½ΠΈΠΈ цСлостности ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… пластов, обСспСчивая мСханичСскоС соСдинСниС ΠΊΠ»Π΅Ρ‚ΠΎΠΊ. ΠŸΡ€ΠΈ ΠΎΠΏΡƒΡ…ΠΎΠ»Π΅Π²ΠΎΠΉ трансформации ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½ΠΎΠ³ΠΎ происхоТдСния Ρ€Π°Π·Ρ€ΡƒΡˆΠ°Π΅Ρ‚ΡΡ ΡΡ‚Π°Π±ΠΈΠ»ΡŒΠ½Π°Ρ мСТклСточная адгСзия. Одним ΠΈΠ· ΡΠ»Π΅Π΄ΡΡ‚Π²ΠΈΠΉ Π½Π°Ρ€ΡƒΡˆΠ΅Π½ΠΈΠΉ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π°Π΄Π³Π΅Π·ΠΈΠΈ являСтся ΠΏΡ€ΠΈΠΎΠ±Ρ€Π΅Ρ‚Π΅Π½ΠΈΠ΅ трансформированными ΠΊΠ»Π΅Ρ‚ΠΊΠ°ΠΌΠΈ способности ΠΊ ΠΈΠ½Π²Π°Π·ΠΈΠΈ. Π’ΠΎ ΠΌΠ½ΠΎΠ³ΠΈΡ… ΠΊΠ°Ρ€Ρ†ΠΈΠ½ΠΎΠΌΠ°Ρ… Π½Π°Π±Π»ΡŽΠ΄Π°Π΅Ρ‚ΡΡ эпигСнСтичСскоС ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠ΅ экспрСссии Π³Π΅Π½Π° Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½Π° БЭН1. Однако описаны Π½Π΅ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ ΠΎΠΏΡƒΡ…ΠΎΠ»ΠΈ ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½ΠΎΠ³ΠΎ происхоТдСния, Π² ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… сохраняСтся экспрСссия Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½Π°. Из ΡΡ‚ΠΎΠ³ΠΎ ΠΌΠΎΠΆΠ½ΠΎ ΡΠ΄Π΅Π»Π°Ρ‚ΡŒ Π²Ρ‹Π²ΠΎΠ΄, Ρ‡Ρ‚ΠΎ мСТклСточная адгСзия ΠΌΠΎΠΆΠ΅Ρ‚ Π½Π°Ρ€ΡƒΡˆΠ°Ρ‚ΡŒΡΡ Π±Π΅Π· подавлСния экспрСссии Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½Π°. ИсслСдования трансформированных ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… Π»ΠΈΠ½ΠΈΠΉ, ΠΊΠ»Π΅Ρ‚ΠΊΠΈ ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… ΡΠΊΡΠΏΡ€Π΅ΡΡΠΈΡ€ΡƒΡŽΡ‚ Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½, ΠΌΠΎΠ³ΡƒΡ‚ ΠΏΡ€ΠΎΡΡΠ½ΠΈΡ‚ΡŒ ΠΌΠ΅Ρ…Π°Π½ΠΈΠ·ΠΌΡ‹ Π½Π°Ρ€ΡƒΡˆΠ΅Π½ΠΈΡ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π°Π΄Π³Π΅Π·ΠΈΠΈ ΠΏΡ€ΠΈ ΠΎΠΏΡƒΡ…ΠΎΠ»Π΅Π²ΠΎΠΉ трансформации.

Π₯ΠΎΡ€ΠΎΡˆΠΎ извСстны различия Π² ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта ΠΈ ΠΠš ΠΊΠ»Π΅Ρ‚ΠΎΠΊ Π΄Π²ΡƒΡ… Ρ‚ΠΊΠ°Π½Π΅Π²Ρ‹Ρ… Ρ‚ΠΈΠΏΠΎΠ²: эпитСлиоцитов ΠΈ Ρ„ибробластов. АК ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠΎΡ†ΠΈΡ‚ΠΎΠ² ΠΎΠ±Ρ€Π°Π·ΠΎΠ²Π°Π½Ρ‹ Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½ΠΎΠΌ, ΠΈΠΌΠ΅ΡŽΡ‚ Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½ΡƒΡŽ ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΡŽ ΠΈ ΡΠ²ΡΠ·Π°Π½Ρ‹ с «ΠΏΠ΅Ρ€ΠΈΡ„СричСским Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²Ρ‹ΠΌ ΠΏΡƒΡ‡ΠΊΠΎΠΌ. ΠΠΎΡ€ΠΌΠ°Π»ΡŒΠ½Ρ‹Π΅ фибробласты ΠΈΠΌΠ΅ΡŽΡ‚ Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½ΡƒΡŽ ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΡŽ АК, ΠΎΡ€ΠΈΠ΅Π½Ρ‚ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… пСрпСндикулярно ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π³Ρ€Π°Π½ΠΈΡ†Π΅ ΠΈ Π°ΡΡΠΎΡ†ΠΈΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… с ΠΏΡ€ΡΠΌΡ‹ΠΌΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²Ρ‹ΠΌΠΈ ΠΏΡƒΡ‡ΠΊΠ°ΠΌΠΈ (Π£ΠΎΠΏΠ΅Ρ‚ΠΈΠ³Π° Π΅Π¬ Π°1., 1995). Π€ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ АК фибробластов сущСствСнным ΠΎΠ±Ρ€Π°Π·ΠΎΠΌ зависит ΠΎΡ‚ ΠΊΠΎΠ½Ρ‚Ρ€Π°ΠΊΡ‚ΠΈΠ»ΡŒΠ½ΠΎΡΡ‚ΠΈ Π°ΠΊΡ‚ΠΈΠ½Π°-ΠΌΠΈΠΎΠ·ΠΈΠ½Π° (Π‘ΠͺΠΈΠ²Π¬Π°ΠΏΠΊΠΎΡƒΠ° et Π°1., 1998, М1ΡƒΠ°ΠΊΠ΅, 2006). ВмСстС с Ρ‚Π΅ΠΌ Π΄ΠΎ ΡΠΈΡ… ΠΏΠΎΡ€ Π½Π΅ Π±Ρ‹Π»ΠΈ исслСдованы закономСрности формирования ΠΠš трансформированных эпитСлиоцитов, Π½Π΅ Π±Ρ‹Π»ΠΈ исслСдованы ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Π΅ взаимодСйствия эпитСлиоцитов, ΡΠΎΡ…Ρ€Π°Π½ΠΈΠ²ΡˆΠΈΡ… ΠΏΡ€ΠΈ трансформации ΡΠΊΡΠΏΡ€Π΅ΡΡΠΈΡŽ Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½Π°. Помимо этого остаётся нСвыяснСнным Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½ΠΎΠ΅ Π·Π½Π°Ρ‡Π΅Π½ΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта, особСнно ΠΏΠΎΡ‚Π΅Ρ€ΠΈ ΠΊΡ€Π°Π΅Π²ΠΎΠ³ΠΎ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΡƒΡ‡ΠΊΠ°, Π² ΠΎΡΠ»Π°Π±Π»Π΅Π½ΠΈΠΈ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π°Π΄Π³Π΅Π·ΠΈΠΈ ΠΏΡ€ΠΈ трансформации эпитСлиоцитов.

На ΡΠΎΠ²Ρ€Π΅ΠΌΠ΅Π½Π½ΠΎΠΌ этапС ΠΎΠ±Ρ‰Π΅ΠΏΡ€ΠΈΠ·Π½Π°Π½Π½ΠΎΠΉ считаСтся Ρ€ΠΎΠ»ΡŒ ΠΌΠ°Π»Ρ‹Ρ… Π“Π’Π€Π°Π· сСмСйства Rho Π² Ρ€Π΅Π³ΡƒΠ»ΡΡ†ΠΈΠΈ пСрСстроСк Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта (Heasman and Ridley, 2008). Π˜ΠΌΠ΅ΡŽΡ‚ΡΡ Ρ‚Π°ΠΊΠΆΠ΅ Π΄Π°Π½Π½Ρ‹Π΅ ΠΎ Π²ΠΊΠ»Π°Π΄Π΅ Rho Π“Π’Π€Π°Π· Π² ΠΏΠΎΡΡ‚Ρ€ΠΎΠ΅Π½ΠΈΠ΅ ΠΠš ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΎΠΊ. Π‘Ρ€Π°Π²Π½ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Π΅ исслСдования Ρ€ΠΎΠ»ΠΈ Π“Π’Π€Π°Π· Rho ΠΈ Rac Π² Ρ‚рансформированных ΠΈ Ρ‚рансформированных эпитСлиоцитах ΠΌΠΎΠ³ΡƒΡ‚ ΠΏΡ€ΠΎΡΡΠ½ΠΈΡ‚ΡŒ ΠΈΡ… Π²ΠΊΠ»Π°Π΄ Π² Ρ€Π°Π·Ρ€ΡƒΡˆΠ΅Π½ΠΈΠ΅ ΡΡ‚Π°Π±ΠΈΠ»ΡŒΠ½ΠΎΠΉ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π°Π΄Π³Π΅Π·ΠΈΠΈ ΠΏΡ€ΠΈ трансформации.

Π’ ΠΏΠΎΡΠ»Π΅Π΄Π½Π΅Π΅ врСмя использованиС Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½Ρ‚Π½Ρ‹Ρ… Π²Π½ΡƒΡ‚Ρ€ΠΈΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… Π±Π΅Π»ΠΊΠΎΠ², ΠΌΠ΅Ρ‡Π΅Π½Π½Ρ‹Ρ… флуорСсцСнтными краситСлями, ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»ΠΎ ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΡ‚ΡŒ исслСдования Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ формирования ΠΠš ΠΈ ΡΡ‚Ρ€ΡƒΠΊΡ‚ΡƒΡ€ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта Π² ΠΆΠΈΠ²Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ…. Π˜Π·ΡƒΡ‡Π΅Π½ΠΈΠ΅ распрСдСлСния флуорСсцСнтно ΠΌΠ΅Ρ‡Π΅Π½Π½Ρ‹Ρ… Π±Π΅Π»ΠΊΠΎΠ² ΠΠš трансформированных итрансформированных эпитСлиоцитов ΠΏΡ€ΠΈ Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚ΠΎΠ² ΠΌΠΎΠΆΠ΅Ρ‚ ΠΏΡ€Π΅Π΄ΠΎΡΡ‚Π°Π²ΠΈΡ‚ΡŒ Π²Π°ΠΆΠ½Ρ‹Π΅ Π΄Π°Π½Π½Ρ‹Π΅ ΠΎ ΡΠ°ΠΌΡ‹Ρ… Ρ€Π°Π½Π½ΠΈΡ… этапах формирования ΠΠš. Π’Π°ΠΊΠΆΠ΅, это ΠΌΠΎΠΆΠ΅Ρ‚ Π²Ρ‹ΡΠ²ΠΈΡ‚ΡŒ различия Π² Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠΉ ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΈ ΠΠšΡ‚рансформированных ΠΈ Ρ‚рансформированных эпитСлиоцитов.

Π’Π°ΠΊΠΈΠΌ ΠΎΠ±Ρ€Π°Π·ΠΎΠΌ, ΠΈΠ·ΡƒΡ‡Π΅Π½ΠΈΠ΅ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ формирования АК, Π΅Ρ‘ ΡΠ²ΡΠ·ΠΈ с ΠΏΠ΅Ρ€Π΅ΡΡ‚Ρ€ΠΎΠΉΠΊΠ°ΠΌΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта ΠΈ ΠΌΠ°Π»Ρ‹ΠΌΠΈ Π“Π’Π€Π°Π·Π°ΠΌΠΈ сСмСйства Rho ΠΈ Π΅Ρ‘ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ Π² Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ нСопластичСской трансформации являСтся ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· Π²Π°ΠΆΠ½Π΅ΠΉΡˆΠΈΡ… Π·Π°Π΄Π°Ρ‡ соврСмСнной ΡΠΊΡΠΏΠ΅Ρ€ΠΈΠΌΠ΅Π½Ρ‚Π°Π»ΡŒΠ½ΠΎΠΉ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΠΈ.

8. Π’Ρ‹Π²ΠΎΠ΄Ρ‹.

1. ΠŸΡ€ΠΈ исслСдовании эпитСлиоцитов Π»ΠΈΠ½ΠΈΠΈ IAR-6−1, трансформированных Π΄ΠΈΠΌΠ΅Ρ‚ΠΈΠ»Π½ΠΈΡ‚Ρ€ΠΎΠ·Π°ΠΌΠΈΠ½ΠΎΠΌ, Π²ΠΏΠ΅Ρ€Π²Ρ‹Π΅ ΠΎΠ±Π½Π°Ρ€ΡƒΠΆΠ΅Π½ΠΎ, Ρ‡Ρ‚ΠΎ нСопластичСская трансформация ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½ΠΎΠ³ΠΎ происхоТдСния ΠΌΠΎΠΆΠ΅Ρ‚ Π²Ρ‹Π·Ρ‹Π²Π°Ρ‚ΡŒ пСрСстройку пространствСнной ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΈ Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½-содСрТащих ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… Π°Π΄Π³Π΅Π·ΠΈΠΎΠ½Π½Ρ‹Ρ… ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚ΠΎΠ² (АК): ΠΏΡ€Π΅Π²Ρ€Π°Ρ‰Π΅Π½ΠΈΠ΅ Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… АК, ассоциированных с ΠΏΠ΅Ρ€ΠΈΡ„СричСским Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²Ρ‹ΠΌ ΠΏΡƒΡ‡ΠΊΠΎΠΌ, Π² Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½Ρ‹Π΅ АК, связанныС с ΠΏΡ€ΡΠΌΡ‹ΠΌΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²Ρ‹ΠΌΠΈ ΠΏΡƒΡ‡ΠΊΠ°ΠΌΠΈ ΠΈ ΠΎΡ€ΠΈΠ΅Π½Ρ‚ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Π΅ пСрпСндикулярно ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π³Ρ€Π°Π½ΠΈΡ†Π΅.

2. ΠŸΡ€ΠΈ нСопластичСской трансформации рСорганизация ΠΠš ΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта сопровоТдаСтся Ρ€Π°Π·Ρ€ΡƒΡˆΠ΅Π½ΠΈΠ΅ΠΌ ΡΡ‚Π°Π±ΠΈΠ»ΡŒΠ½ΠΎΠΉ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π°Π΄Π³Π΅Π·ΠΈΠΈ: Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½-содСрТащиС ΠΠš трансформированных эпитСлиоцитов IAR-6−1 ΡΠ²Π»ΡΡŽΡ‚ΡΡ Π΄ΠΈΠ½Π°ΠΌΠΈΡ‡Π½Ρ‹ΠΌΠΈ структурами ΠΈ ΠΏΡ€Π΅Ρ‚Π΅Ρ€ΠΏΠ΅Π²Π°ΡŽΡ‚ постоянный Ρ€Π΅ΠΌΠΎΠ΄Π΅Π»ΠΈΠ½Π³.

3. ΠΠšΡ‚Ρ€Π°Π½ΡΡ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… ΠΈ Ρ‚рансформированных эпитСлиоцитов Ρ„ΠΎΡ€ΠΌΠΈΡ€ΡƒΡŽΡ‚ΡΡ ΠΏΠΎ-Ρ€Π°Π·Π½ΠΎΠΌΡƒ. ΠΠ°Ρ‡Π°Π»ΡŒΠ½Ρ‹Π΅ Ρ‚ΠΎΡ‡Π΅Ρ‡Π½Ρ‹Π΅ ΠΠšΡ‚рансформированных эпитСлиоцитов ΠΏΡ€Π΅Ρ‚Π΅Ρ€ΠΏΠ΅Π²Π°ΡŽΡ‚ Π»Π°Ρ‚Π΅Ρ€Π°Π»ΡŒΠ½ΠΎΠ΅ Ρ€Π°ΡΡˆΠΈΡ€Π΅Π½ΠΈΠ΅ ΠΈ Ρ„ΠΎΡ€ΠΌΠΈΡ€ΡƒΡŽΡ‚ Π·Ρ€Π΅Π»Ρ‹ΠΉ Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½Ρ‹ΠΉ ΠΠš вдоль ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π³Ρ€Π°Π½ΠΈΡ†Ρ‹. Π’ ΡΠ»ΡƒΡ‡Π°Π΅ трансформированных эпитСлиоцитов, Π½Π°Ρ‡Π°Π»ΡŒΠ½Ρ‹Π΅ Ρ‚ΠΎΡ‡Π΅Ρ‡Π½Ρ‹Π΅ ΠΠš растут ΠΈ ΠΏΡ€Π΅Π²Ρ€Π°Ρ‰Π°ΡŽΡ‚ся Π² ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡƒΠ°Π»ΡŒΠ½Ρ‹Π΅ Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½Ρ‹Π΅ ΠΠš.

4. Π€ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ АК Π² Π½Π΅Ρ‚рансформированных ΠΈ Ρ‚рансформированных эпитСлиоцитах Ρ‚Ρ€Π΅Π±ΡƒΠ΅Ρ‚ активности ΠΌΠ°Π»ΠΎΠΉ Π“Π’Π€Π°Π·Ρ‹ Rho.

5. Π€ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΠš нСтрансформированных эпитСлиоцитов IAR-2 зависит ΠΎΡ‚ ΡΡ„Ρ„Π΅ΠΊΡ‚ΠΎΡ€Π° Rho mDial ΠΈ Π½Π΅ Π·Π°Π²ΠΈΡΠΈΡ‚ ΠΎΡ‚ Π°ΠΊΡ‚ивности ROCK, Π΄Ρ€ΡƒΠ³ΠΎΠ³ΠΎ эффСктора Rho. Π€ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΠš трансформированных эпитСлиоцитов IAR-6−1, Π½Π°ΠΏΡ€ΠΎΡ‚ΠΈΠ², Π½Π΅ Π·Π°Π²ΠΈΡΠΈΡ‚ ΠΎΡ‚ mDial, Π½ΠΎ Ρ‚Ρ€Π΅Π±ΡƒΠ΅Ρ‚ активности ROCK ΠΈ ΠΈΠ½Π΄ΡƒΡ†ΠΈΡ€ΡƒΠ΅ΠΌΠΎΠΉ Сю ΠΊΠΎΠ½Ρ‚Ρ€Π°ΠΊΡ‚ΠΈΠ»ΡŒΠ½ΠΎΡΡ‚ΠΈ Π°ΠΊΡ‚ΠΈΠ½Π°-ΠΌΠΈΠΎΠ·ΠΈΠ½Π°.

6. Π€ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΠš нСтрансформированных эпитСлиоцитов зависит ΠΎΡ‚ Π°ΠΊΡ‚ивности ΠΌΠ°Π»ΠΎΠΉ Π“Π’Π€Π°Π·Ρ‹ Rae. Напротив, Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΠš трансформированных эпитСлиоцитов ΠΌΠΎΠΆΠ΅Ρ‚ ΠΏΡ€ΠΎΠΈΡΡ…ΠΎΠ΄ΠΈΡ‚ΡŒ ΠΈ Π² ΡƒΡΠ»ΠΎΠ²ΠΈΡΡ… ингибирования активности Rae.

7.

Π—Π°ΠΊΠ»ΡŽΡ‡Π΅Π½ΠΈΠ΅

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Π’ Ρ†Π΅Π»ΠΎΠΌ, ΡΡ€Π°Π²Π½ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Π΅ исслСдованиятрансформированных ΠΈ Ρ‚рансформированных эпитСлиоцитов IAR ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, Ρ‡Ρ‚ΠΎ нСопластичСская трансформация ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΎΠΊ Π²Π΅Π΄Ρ‘Ρ‚ ΠΊ Π·Π½Π°Ρ‡ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠΉ Ρ€Π΅ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта, тСсно связанных с Π½ΠΈΠΌ ΠΠš ΠΈ, ΠΊΠ°ΠΊ слСдствиС, ΠΊ Ρ€Π°Π·Ρ€ΡƒΡˆΠ΅Π½ΠΈΡŽ ΡΡ‚Π°Π±ΠΈΠ»ΡŒΠ½ΠΎΠΉ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ Π°Π΄Π³Π΅Π·ΠΈΠΈ. ΠŸΡ€ΠΈ трансформации ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Π΅ ΠΊΠ»Π΅Ρ‚ΠΊΠΈ ΡƒΡ‚Ρ€Π°Ρ‡ΠΈΠ²Π°ΡŽΡ‚ ΠΊΡ€Π°Π΅Π²ΠΎΠΉ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²Ρ‹ΠΉ ΠΏΡƒΡ‡ΠΎΠΊ, Π° ΡΡ‚Π°Π±ΠΈΠ»ΡŒΠ½Ρ‹Π΅ Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½Ρ‹Π΅ ΠΠš Π·Π°ΠΌΠ΅Π½ΡΡŽΡ‚ΡΡ Π΄ΠΈΠ½Π°ΠΌΠΈΡ‡Π½Ρ‹ΠΌΠΈ Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½Ρ‹ΠΌΠΈ ΠΠš. МоТно ΠΏΡ€Π΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠΈΡ‚ΡŒ, Ρ‡Ρ‚ΠΎ отличия ΠΌΠ΅ΠΆΠ΄Ρƒ Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½Ρ‹ΠΌΠΈ ΠΈ Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½Ρ‹ΠΌΠΈ АК, ΠΎΠ±Ρ€Π°Π·ΠΎΠ²Π°Π½Π½Ρ‹ΠΌΠΈ Π•-ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½ΠΎΠΌ, связаны, ΠΏΡ€Π΅ΠΆΠ΄Π΅ всСго, с Ρ€Π°Π·Π»ΠΈΡ‡ΠΈΡΠΌΠΈ Π²ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡ‚Π²ΠΈΠΈ ΠΠšΡ‚рансформированных ΠΈ Ρ‚рансформированных эпитСлиоцитов со ΡΡ‚Ρ€ΡƒΠΊΡ‚ΡƒΡ€Π°ΠΌΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта. ΠŸΡ€ΠΈ Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΠšΡ‚рансформированных эпитСлиоцитов ΠΈΠ³Ρ€Π°Π΅Ρ‚ Ρ€ΠΎΠ»ΡŒ полимСризация F-Π°ΠΊΡ‚ΠΈΠ½Π°, рСгулируСмая mDial ΠΈ Rae. Π’ Ρ‚ΠΎ ΠΆΠ΅ врСмя Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΠš трансформированных эпитСлиоцитов опрСдСляСтся ΠΈΡ… Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡ‚Π²ΠΈΠ΅ΠΌ с Π°ΠΊΡ‚ΠΈΠ½-ΠΌΠΈΠΎΠ·ΠΈΠΏΠΎΠ²Ρ‹ΠΌΠΈ ΠΏΡƒΡ‡ΠΊΠ°ΠΌΠΈ, ΠΊΠΎΠ½Ρ‚Ρ€Π°ΠΊΡ‚ΠΈΠ»ΡŒΠ½ΠΎΡΡ‚ΡŒ ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… рСгулируСтся ROCK.

По ΠΈΡ‚ΠΎΠ³Π°ΠΌ настоящСй Ρ€Π°Π±ΠΎΡ‚Ρ‹ сформулирована Π³ΠΈΠΏΠΎΡ‚Π΅Π·Π°, ΠΏΡ€Π΅Π΄ΠΏΠΎΠ»Π°Π³Π°ΡŽΡ‰Π°Ρ, Ρ‡Ρ‚ΠΎ пСрСстройка Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΠš ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΎΠΊ Π² Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½Ρ‹Π΅ ΠΠš Π²ΠΎ Π²Ρ€Π΅ΠΌΡ нСопластичСской трансформации опрСдСляСтся Ρ€Π΅ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠ΅ΠΉ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта, приводящСй ΠΊ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡΠΌ Π² Π½Π°ΠΏΡ€Π°Π²Π»Π΅Π½ΠΈΠΈ натяТСния Π² Π·ΠΎΠ½Π΅ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠ³ΠΎ ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚Π°. Π˜ΡΡ‡Π΅Π·Π½ΠΎΠ²Π΅Π½ΠΈΠ΅ ΠΊΡ€Π°Π΅Π²ΠΎΠ³ΠΎ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΡƒΡ‡ΠΊΠ° Π² ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… ΠΏΡ€ΠΈ трансформации являСтся ΠΊΠ»ΡŽΡ‡Π΅Π²Ρ‹ΠΌ событиСм, приводящим ΠΊ ΠΏΠ΅Ρ€Π΅ΡΡ‚Ρ€ΠΎΠΉΠΊΠ΅ ΠΠš. Π’ Ρ‚рансформированных ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… отсутствиС Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½ΠΎΠ³ΠΎ натяТСния Π² Π·ΠΎΠ½Π΅ ΠΌΠ΅ΠΆΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠ³ΠΎ ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚Π° прСпятствуСт Ρ€Π°Π·Π²ΠΈΡ‚ΠΈΡŽ ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚Π½ΠΎΠ³ΠΎ ΠΏΠ°Ρ€Π°Π»ΠΈΡ‡Π°, Π° Π½Π°Π»ΠΈΡ‡ΠΈΠ΅ Ρ†Π΅Π½Ρ‚Ρ€ΠΎΡΡ‚Ρ€Π΅ΠΌΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠ³ΠΎ натяТСния ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ ΠΊ ΡΠ±ΠΎΡ€ΠΊΠ΅ прямых Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²Ρ‹Ρ… ΠΏΡƒΡ‡ΠΊΠΎΠ² ΠΈ ΠΎΠ±Ρ€Π°Π·ΠΎΠ²Π°Π½ΠΈΡŽ Ρ€Π°Π΄ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… АК, ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ Π³ΠΎΡ€Π°Π·Π΄ΠΎ Π΄ΠΈΠ½Π°ΠΌΠΈΡ‡Π½Π΅Π΅ ΠΈ ΠΌΠ΅Π½Π΅Π΅ ΡΡ‚Π°Π±ΠΈΠ»ΡŒΠ½Ρ‹, Ρ‡Π΅ΠΌ Ρ‚Π°Π½Π³Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½Ρ‹Π΅ ΠΠšΡ‚рансформированных ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΎΠΊ. Π’Π°ΠΊΠΈΠ΅ ΠΊΠΎΠ½Ρ‚Π°ΠΊΡ‚Ρ‹ ΠΌΠΎΠ³ΡƒΡ‚ ΠΈΠ³Ρ€Π°Ρ‚ΡŒ Π²Π°ΠΆΠ½ΡƒΡŽ Ρ€ΠΎΠ»ΡŒ Π² ΠΊΠΎΠ»Π»Π΅ΠΊΡ‚ΠΈΠ²Π½ΠΎΠΉ ΠΌΠΈΠ³Ρ€Π°Ρ†ΠΈΠΈ трансформированных эпитСлиоцитов. ИзмСнСния Π² Π°ΠΊΡ‚ивности ΠΌΠ°Π»Ρ‹Ρ… Π“Π’Π€Π°Π· сСмСйства Иго, ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ Ρ…Π°Ρ€Π°ΠΊΡ‚Π΅Ρ€Π½Ρ‹ для трансформированных ΠΊΠ»Π΅Ρ‚ΠΎΠΊ, Ρ‚Π°ΠΊΠΆΠ΅ ΠΌΠΎΠ³ΡƒΡ‚ Π²Π½ΠΎΡΠΈΡ‚ΡŒ Π²ΠΊΠ»Π°Π΄ Π² ΠΏΠ΅Ρ€Π΅ΡΡ‚Ρ€ΠΎΠΉΠΊΠΈ ΠΠš ΠΈ Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ цитоскСлСта. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±Ρ€Π°Π·ΠΎΠΌ, настоящая Ρ€Π°Π±ΠΎΡ‚Π° дСмонстрируСт Ρ‚ΠΎ, Ρ‡Ρ‚ΠΎ структурная ΠΈ Π΄ΠΈΠ½Π°ΠΌΠΈΡ‡Π΅ΡΠΊΠ°Ρ координация ΠΌΠ΅ΠΆΠ΄Ρƒ ΠΊΠ°Π΄Ρ…Π΅Ρ€ΠΈΠ½Π°ΠΌΠΈ, Π°ΠΊΡ‚ΠΈΠ½ΠΎΠ²Ρ‹ΠΌΠΈ структурами ΠΈ Π°ΠΊΡ‚ΠΈΠ½-Ρ€Π΅Π³ΡƒΠ»ΠΈΡ€ΡƒΡŽΡ‰ΠΈΠΌΠΈ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ ΠΌΠΎΠΆΠ΅Ρ‚ ΠΈΠ·ΠΌΠ΅Π½ΡΡ‚ΡŒΡΡ ΠΏΡ€ΠΈ нСопластичСской трансформации ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΎΠΊ.

ΠŸΠΎΠΊΠ°Π·Π°Ρ‚ΡŒ вСсь тСкст

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