Взаимодействие рибосомного белка L1 с рибосомной и матричной РНК

выводы.

1. Получены пригодные к рентгеноструктурному анализу кристаллы комплекса рибосомного белка М]аЬ1 с фрагментом его мРНК, и двух комплексов рибосомного белка ТЧЫЛ с разными по длине фрагментами архейной мРНК, кодирующей белок Муа Ы.

2. Определены пространственные структуры комплекса муаЫ-мРНК^аи с разрешением 3.4 и комплекса ТЧЫЛ-мРНКмуаы с разрешением 2.6 (совместно с группой структурных исследований рибосомных белков).

3. Получены мутантные формы белков М]а1Л и ТЧЫЛ с заменами аминокислотных остатков, вовлеченных в РНК-белковые взаимодействия, и измерены константы связывания этих белков с мРНК и рРНК. Подтверждено выдвинутое ранее предположение о ключевой роли кластера консервативных аминокислотных остатков первого домена белка Ы в специфическом связывании с РНК.

Подтверждены выводы, сделанные из сравнительного анализа структур комплексов Ы-рРНК и Ы-мРНК, об определяющей роли домена II белка Ы в обеспечении повышенного сродства этого белка к рРНК по сравнению с его сродством к мРНК.

4. Получены кристаллы ряда мутантных форм белка Ь1, что позволило определить пространственные структуры пяти из этих мутантных белков.

Показано, что замены полярных остатков на аланин не приводят к значительным изменениям рельефа поверхности белка Ь1, контактирующей с РНК.

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186. Выражаю искреннюю благодарность моему научному руководителю, Марине Борисовне Гарбер, за чуткое руководство и внимание к моей работе, а так же за ценные советы и рекоммендации, сделанные в процессе подготовки диссертации.

187. Приношу свою глубокую благодарность Светлане Викторовне Тищенко за огромную помощь в освоении различных биохимических методов, за обучение методам кристаллизации, за ценнейшие советы и постоянную поддержку во время выполнения этой работы.

188. Очень признательна всем сотрудникам группы структурных исследований рибосомных белков за помощь в совместной работе и обсуждении полученных результатов.

189. Благодарю всех сотрудников лаборатории структурных исследований аппарата трансляции за дружескую поддержку.