Функциональная топография транспортно-матричной РНК

выводы.

1. Элонгационный фактор Tu и тмРНК участвуют во взаимодействии нового типа. В отличие от канонического комплекса между ацилированным тРНК-подобным доменом тмРНК и EF-Tu*GTP деацилированная тмРНК образует комплекс с EF-Tu*GDP за счёт взаимодействия со спиралью 2 и псевдоузлом 4.

2. Белок SrapB входит в состав тмРНК-рибосомных комплексов на всех стадиях транс-трансляции. Элонгационные комплексы содержат по одной молекуле белка SmpB, связанной с каноническим участком взаимодействия на TLD тмРНК.

3. Молекула тмРНК в растворе существует в виде двух компактно свернутых доменов, между которыми уложена одноцепочечная мРНК-подобная область. В ходе транс-трансляции домены, не изменяя своей структуры, занимают определённые сайты на рибосоме, в то время как матричная область перемещается по ней в экспонированной форме.

4. Экспериментальная проверка двухдоменной модели с помощью химических, физических и биоинформационных подходов позволяет построить динамическую модель транс-трансляции, адекватную всей совокупности имеющихся на' сегодняшний день данных. Согласно этой модели структурные элементы тмРНК (псевдоузлы, спирали, арка вокруг головы 30S рибосомной субчастицы, сформированная псевдоузлами), сохраняются в процессе шранотрансляции. Основные изменения при прохождении MLD тмРНК через рибосому затрагивают район мРНК-подобной области и спирали 5 тмРНК.

5. Правильное позиционирование ко дона возобновления синтеза в А-участке рибосомы задается структурным элементом, сформированным белком SmpB, псевдоузлом 1 и петлёй А79-А86 тмРНК, который совершает поворот при транслокации TLD тмРНК из А-участка рибосомы в Р-участок.

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