Оптические биосенсоры для определения фенольных соединений и органических пероксидов

выводы.

1. Разработан способ получения прозрачных, механически прочных и каталитически активных пленок состава {пероксидаза-хитозан}, {тирозиназа-хитозан}, {лакказа-хитозан}, сохраняющих свои свойства после выдерживания в водных и водно-органических средах (в присутствии до 40% ДМСО, ацетонитрила, этанола).

2. Предложена новая индикаторная система: взаимодействие продуктов ферментативного окисления фенолов с хитозаном с образованием ковалентно связанного с ним светопоглощающего аддукта, позволяющая регистрировать аналитический сигнал непосредственно в матрице биочувствительного слоя вне анализируемого раствора.

3. На основе полученных пленок и предложенной индикаторной системы созданы оптические биосенсоры простой конструкции для определения фенольных соединений и органических пероксидов в объектах со сложной матрицей без предварительной пробоподготовки.

4. В результате изучения влияния на чувствительность и селективность биосенсоров состава их чувствительного слоя, рН, концентрации, природы буферного раствора и фермента, температуры, содержания полярного органического растворителя (ДМСО) разработаны методики определения в водных или водно-органических средах растворимых и нерастворимых в воде фенольных соединений и органических пероксидов в диапазонах концентраций, соответственно: гидрохинона и пирокатехина — 20−200 и 25−250 мкМкверцетина, рутина, эскулетина — 10−200, 10−150, 10−150 мкМ- 2-бутанонпероксида и бензоилпероксида — 0.05−1 и 0.05−25 мМ.

5. Продемонстрирована возможность применения сенсора, созданного на основе пленки {пероксидаза-хитозан}, для анализа реальных объектов (крема, геля, витамина в таблетках и порошка для приготовления инъекций) без отделения матрицы и полной гомогенизации анализируемого раствора, а также в присутствии органического растворителя ДМСО.

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