Конструирование рекомбинантных плазмид, содержащих ген фибера вируса ССЯ-76 и изучение иммунного ответа лабораторных животных на введение этих плазмид: Генетическая иммунизация

ВЫВОДЫ.

1. Впервые был получен набор рекомбинантных плазмид, содержащих, ген фибера вируса ССЯ-7 6 под контролем различных промоторов.

С помощью метода направленного мутагенеза были получены рекомбинатные плазмиды, содержащие нуклеотидные замены в вариабельной области гена фибера.

2. Впервые показано, что инъекция рекомбинантными плазмидами, содержащими ген фибера ССЯ-7 6 белым мышам (генетическая иммунизация), вызывает образование клеточного и гуморального иммунного ответа.

3. Разработаны схемы генетической иммунизации лабораторных животных рекомбинантными плазмидами, содержащими ген фибера вируса ССЯ-7 6.

4. Установлено наличие вируснейтрализующих антител (титр 4−51од2)в сыворотках крови лабораторных животных, подвергнутых генетической иммунизации рекомбинантными плазмидами.

5. Впервые проведен синтез изолированного белка фибера вируса ССЯ-7 6 в бакуловирусной системе, а также в культуре клеток млекопитающих и птиц, определена молекулярная масса рекомбинатного белка и изучены его антигенные свойства.

1. Ануфриева Т. А., Ольшевская Т. И. Синдром Снижения Яйценоскости кур. (Обзор литературы) ВНИИЗЖ, Владимир, 1992;

2. Бакулин В. А., Хохлачев О. Ф., Савостьянов Г. А. и др. Патоморфология при болезни птиц ССЯ-7 6(синдром снижения яйценоскости)//Ветеринария 1988 № 6 стр. 28−31;

3. Березин В. Э. // 198 6 Вопр. Вирусологии № 3 стр 2 62;

4. Бессарабов Б. Ф. Методические рекомендации профилактика синдрома снижения яичной продуктивности (сся-7 6) Москва 1997.

5. Бурцев В. И., Бесхлебнов В. А., Козлова Д. И. Синдром снижения яйценоскости. Вопросы ветеринарной вирусологии, микробиологии и эпизоотологии. //Тезисы докл. научной конф. Покров. 1983 стр. 61−66;

6. Дебабов В. Г. ДНК-вакцинация и генотерапия на основе транзиетной экспрессии нуклеиновых кислот в соматических клетках человека и животных. // Молекулярная биология 1997 т. 31№ 1 стр. 209−215;

7. Иммуноферментный анализ. П/р Т. Нго и Г. Ленхоффа // М. Мир 1988 стр. 193−209. ;

8. Коровин Р. Н., Зелинский В. П, Трошева Г. А. Синдром снижения яйценоскости-76//Лабораторная диагносттика болезней птиц: Справочник. -М: Агропромиздат 1989 с98−99;

9. Лярски 3. Диагностика вирусных болезней животных. // М. Колос 198 0 г;

10. Маниатис Т., Фрич, Сэмбрук Д. Молекулярное клонирование. // -М.: Мир. -198 4;

11. Мейхи Б. Вирусология. Методы. //М. Мир 198 8 г. ;

12. Рождественский И. К. Инактивация вируса ССЯ-7 6 // Ветеринария 1984 № 4 стр. 61−62.

13. Рождественский И. К., Коровин Р. Н., Седунов В. А. Синдром снижения яйценоскости-аденовирусная болезнь кур. //Ветеринария 1981 № 4 стр. 70−71;

14. Смоленский В. И. Средства и методы специфической профилактики болезней птиц вирусной этиологии. Автореф. докт. биолог, наук. М. 1999.

15. Соколов Е. И. //19 98 М. Клиническая Иммунология;

16. Суни Ю. Иммуноферментный метод (ИФА) при вирусологической диагностике: Метод, обзор фирмы «Лабсистемс Ой» (Финляндия). -Турку, 1981. ;

17. Сюрин В. Н., Белоусова Р. В., Фомина Н. В. Диагностика вирусных болезней животных: Справочник. М.: Агропромиздат. 1991 стр. 159−166;

18. Ханаан Д. Методы трансформации. Клонирование ДНК. -М. -1988. -С. 140−173.

19. Фомина Н. В. Аденовирусная инфекция животных в 2х т. Т2 М. Колос 1995 193 с;

20. Abai А. М., Kuwahara-Rundell А., Margalith М., Morrow J., et all. Coinjection of the genes fo interleukin-2 and antigen does not increase the humoral immune response. In Vaccines 1994(Norby E., Brown J. A., Chanock R., Ginsberg R. S, eds) pp 77−81;

21. Adair В. M., McFerran J. В., Connor T. J., McNulty M. S., McKillop E. R. Biological and physical properties ofa virus (strain 127) associated with egg drop syndrome 1976 //Avian. Pathol. 1979 v8 pp 249−256;

22. Adair B. M., Todd D., McFerran J. B., McKillop S. R. Comparative serological studies with egg drop syndrome virus. // Avian. Pathol. 1986 № 15 pp 677−685;

23. Bai M, Harfe B, Freimuth P. Mutations that alter an Arg-Gly-Asp (RGD)sequence in the adenovirus type 2 penton base protein abolish its cell-rounding activity and delay virus reproduction in flat cells. //J Virol 1993 Sep- 67 (9): 5198−205;

24. Baxendale W. Egg drop syndrome. //Vet Rec. 1978 May 20- 102 (20) :450;

25. Baxendale W., Latticken D., Hein R., Observations made with the egg drop syndrome-76 of chickens and the development of a vaccine against disease// Proc. 26th World’s Poultry Congress and Exposition. Rio Da Janeiro, 1978 pp 117−120;

26. Baxendale W. Convencion annal de la asociacion Nacional de espesialiatas on cicuus Avicolas//Recent researc on egg drop syndrome-76(EDS-76)1980 pp 206−211;

27. Baxendale W., Latticken D., Hein R., McPherson L. The results of field trials conducted with ana inactivated vaccine against egg drop syndrome 76(EDS76)//Avian. Pathol 1983 № 12 pp. 9−16;

28. Beard CW, Mason PW. Out on the farm with DNA vaccines. //Nat. Biotechnol 1998 Dec- 16(13)pp. 1325−8;

29. Borgen HC, Espensen L. Immunizing effect of photoinactivated pseudorabies virus. //Zentralbl Bakteriol OrigJ 1970 v214(l)pp. 13−6;

30. Boudin MR. Isolation and characterization of adenovirus type 2 vertex capsomer (penton base). // Virology. 1979 Jan 15 v92(l)pp. 125−38;

31. Brugere Picoux J. Les adenovirus en pathologie aviare// Rec. Med. Veter. 1978 vl54. Pp. 1015−1021;

32. Carbone FR, Bevan MJ. Class I-restricted processing and presentation of exogenous cell-associated antigen in vivo. //J Exp. Med. 1990 Feb 1 v. 171(2)pp. 377−87;

33. Chalguest R. R. A Statistical model to optimize indirect Sandwich Enzyme-Linked immunosorbent assay parameters of antigen and antibody: a microcomputer program. // Avian Diseases 1987 v31 pp. 509−512;

34. Chow Y. H., Chiang B. L., Lee Y. L. et all. Development of Thl and Th2 populations and the nature of immune responses to hepatitis B virus DNA vaccines can be modulated by codelivery of various cytokine genes. // J. Immunol. 1998 160 pp. 1320−1323;

35. Cohen A. D., Boyer J. D., Weiner D. B. Modulating the immune response to genetic immunization. //1998 FASEB lett. vl2 pp. 1611−1626;

36. Cook J. K. Egg drop syndrome 1976 (EDS 1976) virus infection in adequately vaccinated chickens// Avian Pathol 1983 № 12 pp. 9−16;

37. Darbyshire J. H, Peters R. W. Studies on EDS 76 virus infection in laying chikens. //Avian. Pathol. 1980 v9 pp. 277−290;

38. Davis HL, Weeratna R, Waldschmidt TJ, Tygrett L, Schorr J, Krieg AM. CpG DNA is a potent enhancer of specific immunity in mice immunized with recombinant hepatitis B surface antigen. //J. Immunol 1998 Jan 15v. 160(2)pp. 870−6;

39. Devaux C, Zulauf M, Boulanger P, Jacrot B. Molecular weight of adenovirus serotype 2 capsomers. A new characterization. // J. Mol. Biol. 1982 № 9 pp. 927−939;

40. Devaux C., Caillet-Boudin M. L., Jacrot B., Boulanger P. Crystallisation, enzymatic clevage, and the polarity of the adenovirus type 2 fiber. // Virology 1987 v 161 pp. 121−128;

41. Devaux C., Adrian M., Berthet-Colominas C. et all. Structure of adenovirus fiber. I. Analysis of crystals from adenovirus serotypes 2 and 5 by electron microscopy and X-ray crystallography. // J. Mol. Biol. 1990 v 215 pp. 5 67−5 68;

42. Davis H. L, Whalen R. G. DNA-based immunization. // Mol. Cell Biol. Hum. Dis. Ser. 1995 v. 5 pp. 368−87;

43. Davis H. L, Whalen R. G, Demeneix BA. Direct gene transfer into skeletal muscle in vivo: factors affecting efficiency of transfer and stability of expression. // Hum. Gene Ther. 1993 Apr v. 4(2)pp. 151−9;

44. Donelly J. J., Ulmer J. B., Shiver J. W., Liu M. A. DAN vaccines. //Annu. Rev. Immunol. 1997 v 15 pp 61 764 8;

45. Dorner F, Eibl J, Barrett PN. New technologies for vaccines. //Wien Klin. Wochenschr. 1999 Mar 12 v. Ill (5)pp. 199−206;

46. Dummer R, Davis-Daneshfar A, Dohring C, Dobbeling U, Burg G. Strategies for gene therapy of melanoma // Hautarzt 1995 May v. 46(5)pp. 305−8;

47. Eck J. H. Serological examination and egg production of fowl experimentally infected with egg drop syndrome. //1976 virus Vet Q. 1982 № 4 pp. 117−121;

48. Engvall E, Perlman P. Enzyme-linked immunosorbent assay (ELISA). Quantative assay of immunoglobulin G. // Immunochemistry 1990 v8 pp. 871−874;

49. Ertl H. C, Xiang Z. Q. Genetic immunization. // Viral Immunol 1996 v. 9 (1)pp. 1−9;

50. Feltquate D. M, Heaney S, Webster R. G, Robinson H. L. Different T helper cell types and antibody isotypes generated by saline and gene gun DNA immunization. // J Immunol 1997 Mar 1 vl58(5)pp. 2278−84;

51. Fenner F. The classification and nomenclature of viruses. // Intervirology-1975 v. 1 № 1 pp. 1−13;

52. Fynan E. F, Webster R. G, Fuller D. H, Haynes J. R, Santoro J. C, Robinson H. L. DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations. // Proc. Natl. Acad. Sci. USA 1993 Dec 15 v. 90 (24)pp. 11 478−82;

53. Gelderblom H., Maichle-Lauppe I. The fibers of fowl adenovirus // Arch. Virol. -1982. -Vol. 72. -pp. 289−295;

54. Ginsberg H. S., Pereira H. G., Valentine R. C, Wilcox W. C. A proposed terminology for the adenovirus antigens and virion morphological subunits//Virology 1966 v. 28 pp. 782−783;

55. Gough R. E, Collins M. S., Spackman D. Isolation of haemagglutinating adenovirus from commercial ducks. //Vet Rec. 1982 v. 110 pp. 275−276;

56. Green N. M., Wrigley N. G., Russell W. G., Martin S. R., McLachlan A. S. Evidency for a pepeating cross-sheet structure in the adenovirus fibre. //EMBO. 1983 v. 2 pp. 1357−1365;

57. Hamblin C, Barnett I. T. R. r Hedger R. S. A new enzyme-linked immunosorbent assay (ELISA)for detection of antibodies against FMD virus 1. Development and method of ELISA. //Immunol. Methods 1986 v. 93 pp. 115−121;

58. Hartikka J., Sawdey M., Cornefert-Jensen F., et all. An improved plasmid DNA expression vector for direct injection into skeletal muscle. Hum. Gene Ther. 1996 Jun 20 v. 7(10)pp. 1205−17;

59. Hasan U. A., Abai A. M., Harper D. R. et all. Nucleic acid immunization: concepts and techniques associated with third generation vaccines. // J. Immunol. Methods. 1999 v. 229 pp. 1−22;

60. Hassett D. E., Whitton. J. L. DNA immunization. // Trends in Mcrobiology 1996 v. 4 № 8 pp. 307−311;

61. Higashihara M., Takai S., Hidako A. et all. Isolation of the virus of egg drop syndrome 1976(EDS76)in Japanese outbreak// Jap. J. Vet. Sci. 1983 v. 45 pp. 603 612;

62. Hilmes H. C. Webb K. J., Box F. G. Vaccine for the control egg drop syndrome 1976//Vet Rec. 1989 v. 124 pp. 309−310;

63. Hess M., Blocker H., Brandt P. The complete nucleotide sequence of the egg drop syndrome virus: an intermediate between Mastadenoviruses and Aviadenoviruses // Virology 1997 v. 238. pp. 145−156;

64. Heufler C, Koch F, Schuler G Granulocyte/macrophage colony-stimulating factor and interleukin 1 mediate thematuration of murine epidermal Langerhans cells into potent immunostimulatory dendritic cells. //.

65. J. Exp. Med. 1988 Feb 1 v. 167(2)pp. 700−5;

66. Hohlfeld R., Engel A. G. The immunology of muscle. //Immunol. Today 1994 v. 15 pp. 269−274;

67. Holmes D., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. // Anal. Biochem., 1981, v. 114, pp. 193.

68. Hong J. S., Engler J. A. The amino terminus of the adenovirus fiber protein encides the nuclear localization signal. //Virology 1991 v. 185 pp. 758−767;

69. Hong J. S., Engler J. A. Domains required for assembly of adenovirus type 2 fiber trimers. // J. Virology 1996 pp. 7071−7078;

70. Hong S. S., Karayan L., Tournier J. et all. Adenovirus type 2 fiber knob binds to MHC class I domain at the surface of human epithelial and B lymphoblastoid cells. // EMBO 1997 v. 16 № 9 pp. 2294−2306;

71. Huang S., Reddy V., Dasgupta N., Nemerow G. R. A single amino acid in the adenovirus type 37 fiber confers binding to human conjunctival cells. // J. Virology 1999 pp. 2798−2802;

72. Huang A. Y., Golumbek P., Ahmadzadeh M., Jaffee E., Pardoll D., Levitsky H. Role of bone marrow-derived cells in presenting MHC class I-restricted tumor antigens. // Science 1994 May 13 v. 264(5161)pp. 961−5;

73. Iwasaki A., Torres C., Ohashi P. S., et all. T he dominant role of bone marrow-derived cells in CTL induction following plasmid DNA immunization at different sites. // J. I mmunol. 1997 v. 159 pp. 11−14;

74. Janeway C. A., Travers P. Immunobiology: The immune system in health and disease. //1994 7:31—7:32, Current Biology Ltd., London;

75. Karayan L., Gay B., Gerfaux J., Boulanger P. A. Oligomerization of recombinant penton base ofadenovirus type 2 an its assembly with fiber in baculovirus-infected cells. // Virology 1994 v. 202 pp. 782−795;

76. Kim J. J., Ayavoo V., Bagarazzi M. L., et all In vivo engineering of a cellular immune response by coadministration of IL-12 expression vector with a DNA immunogen // J. Immunol 1997 Jan 15 v. 158(2)pp. 816−26.

77. King DJ. Evaluation of different methods of inactivation of Newcastle disease virus and avian influenza virus in egg fluids and serum. // Avian Dis. 1991 Jul-Sep v. 35(3)pp. 505−14;

78. Kissary J, Zsak L The molecular weight of the egg drop syndrome (EDS)avian adenovirus (strain B8/78)DNA estimated by digestion with restriction endonuclease enzyme EcoR I //Arch. Virol 1980 v. 65 pp. 63−65;

79. Klinman D. M., Yamshchikov G., Ishigatsubo Y. Contribution of CpG motifs to the immunogenicity of DNA vaccines. // J Immunol 1997 Apr 15 v. 158(8)pp. 3635−9;

80. Kohler H, Loupai G Zum Egg Drop Syndrome des Huhnes. 2 Mittilunge, Pathologische, Anatomiz und Histopatholoie // Dt. tierarztl. Wschr. 1982. Ed89. S14−16;

81. Krasnykh V. N., Mikheeva G. V., Douglas J. T., Curiel D. T. Generation of Recombinant adenovirus vectors with modified fibers for altering viral tropism. // J. Virology 1996 pp. 6839−6846;

82. Krasnykh V. N, Dmitriev I., Mikheeva G. et all. Characterization of ana adenovirus vector containing aheterologous peptide epitope in the HI loop of the fiber protein. // J. Virology 1998 pp. 1844−1852;

83. Krieg A. M. An innate immune defense mechanism based on the recognition of CpG motifs in microbial DNA. // J. Lab. Clin. Med. 1996 Aug v. 128(2)pp. 128−33;

84. Krieg A. M., Yi A. K., Matson S., et all. CpG motifs in bacterial DNA trigger direct B-cell activation. // Nature 1995 Apr 6v. 374(6522)pp. 546−9;

85. Kumar R, Mohanty K. C., et all. Epi zootiological studies on egg drop syndrome in poultry// Indian J. Anim. Sei. v. 62 pp. 497−501;

86. Laver W. G., Suriano J. R., Green M. Adenovirus proteins. 2. N-terminal amino acis analysis// Virology 1967 v. 1 pp. 723−728;

87. Lee L. F., Witter R. L. Humoral immune responses to inactivated oil-emulsified Marek’s disease vaccine. // Avian Dis. 1991 Jul-Sepv v. 35(3)pp. 452−9;

88. Liang X., Hartikka J., Sukhu L., Manthorpe M., Hobart P. Novel, high expressing and antibiotic-controlled plasmid vectors designed for use in gene therapy. // Gene Ther. 1996 Aprv. 3 (4)pp. 350−6-89. Lieberman.

89. Liu M. A., McClements W., Ulmer J. B., Shiver J., Donnelly J. Immunization of non-human primates with DNA vaccines. //Vaccine 1997 Jun v. 15(8)pp. 909−12;

90. Lu Y. S., Lin D. F., Tsai H. J. Outbreaks of egg drop syndrome-1976 in Taiwan and isolation of the etiological agent. //J. Clin. Soc. Vet. Sci. 1985 № 11 pp. 157−165;

91. Ma S. H., Tan J. M., Wu Z., Tal X. G. Detection of the antibody of avian egg drop syndrome-76 by using the competition enzyme linked immunosorbent assay// J. of Shanghai Agriculrure college 1991 v. 9 pp. 285−289;

92. Malkinson M., Weisman Y. // Serological survey for the prevelance of antibodies to egg drop syndrome 1976 virus in domesticated and wild birds in Israel. //Avian Pathol. 1980 v. 9 pp. 421−426;

93. McCracken R. M., McFerran J. B. Experimental reproduction of the egg drop syndrome 7 6 with heamagglutinating adenovirus //Avian. Pathol. 1978 v. 7 pp. 483−490;

94. McFerran J. B., Connor T. J., McCracken R. M. Isolation of adenoviruses and reoviruses from avian species other than domestic fowl // Avian Dis. 1976 v. 20. pp. 519−526;

95. McFerran J. B., Rowley, Helen M. Serological studies on flock showing depressed egg drop production//Avian. Pathol 1977 v. 6 pp. 405−413;

96. McFerran J. B., McNulty M. S., Curran W. L. Diagnosis of avian disease by electron microscopy// Am. J. Vet. Res. 1978 v. 39 pp. 505−508;

97. McFerran J. B., McCracken R. M. et all. Studies of depressed egg production syndrome in Northern Ireland. // Avian. Pathol. 1978 № 7 pp. 35−47;

98. McFerran J. B. Egg drop syndrome. // Disease of Poultry. Iova State University, Iowa, USA 1997 pp. 5525 63;

99. Morin N., Boulanger P. Morphogenesis of human adenovirus type 2: sequence of entry of proteins into previral and viral particles. //Virology 1984 Jul 15 v. 13 6(1)pp. 153−67;

100. Morrissey P. J., Bressler L., Park L. S. et all. Granulocyte-macrophage colony-stimulating factor augments the primary antibody response by enhancing the function of antigen-presenting cells. // J. Immunol 1987 Aug 15 v. 13 9(4)pp. 1113−9;

101. Nichols W. W., Ledwith B. J., Manam S. V., Troilo P. J. Potential DNA vaccine integration into host cell genome. // Ann N Y Acad Sci 1995 Nov 27 v. 772 pp. 30;

102. Normann J. A., Hobart P., Manthorpe M., Feigner P., Wheeler C. Development of improved vectors for DNA-based immunization and other gene therapy applications. //Vaccine 1997 pp. 801−803;

103. Novell! A., Boulanger P. A. Deletion analysis of structural domains in baculovirus-expressed adenovirus type 2 fiber. // Virology 1991 pp. 365−376;

104. Novelli A., Boulanger P. Assembly of adenovirus type 2 fiber synthesized in cell-free translation system. // J. Biol. Chem 1991 v. 266 pp. 365−376;

105. Ogra P. L., Faden H. S., Abraham R. et all. Effect of prior immunity on the shedding of virulent revertant virus in feces after oral immunization with live attenuated poliovirus vaccines. //J. Infect. Dis. 1991 Jul v. 164(l)pp. 191−4;

106. Pardoll D. M., Beckerleg A. M. Exposing the immunology of naked DNA vaccines. //Immunity 1995 Aug-v. 3(2)pp. 165−9;

107. Martins L. P., Lau L. L., Asano M. S., Ahmed R. DNA vaccination against persistent viral infection. // J. Virology 1995 pp. 2574−2582;

108. Petterson U., Wadell G. Antigen structure of the adenoviruses // Immunochemistry of Viruses / Eds. Van Regenmortel and Neirath A. R. -Elsevier Sci: Publishers, B. V. 1985. pp. 295−323;

109. Pertmer T. M., Eisenbraun M. D., McCabe D. et all. Gene gun-based nucleic acid immunization: elicitation of humoral and cytotoxic T lymphocyte response folowing epidermal delivery of nanogramm quantities of DNA. //Vaccine 1995 pp. 1427−1430;

110. Philipson L. 1983 Structurel and assembly adenovirus" Curr. Topics in Microbiology ana Immunology 1983 v. 109;

111. Prayaga S. K., Ford M. J, Haynes J. R. Manipulation of HIV-1 gpl20-specific immune responses elicited via gene gun-based DNA immunization. //Vaccine 1997 Aug-Sep v. 15(12−13)pp. 1349−52;

112. Raz E., A Watanabe, S. M. Baird, R. A. Eisenberg, T, B. Parr, M. Lotz, T. J. Kipps, and D. A. Carson Systematic Immunological Effects of Cytokine Genes Injected into Skeletal Muscle. // Proc. Natl. Acad. Sci 1993 v. 90 pp. 4523−4527;

113. Russels W. C., Adrian T, Bartha A. Classification and Nomenckature of viruses. Sixth report of the international Commitee on «Taxonomy of viruses'// Virus Taxonomy-Wien, New York, 1995 pp. 128−133;

114. Salmon J. K., Armstrong C. A, Ansel J. C. The skin as an immune organ. // West. J. Med. 1994 Feb v. 160(2)pp. 146.

115. Sanford J. C, Smith F. D., Russell J. A. Optimizing the biolistic process for different biological applications. // Methods Enzymol. 1993 v. 217 pp. 483 509;

116. Schloer G. M. Frequency of antibody to adenovirus 127 120. in domestic ducks and wild waterfowl // Avian Dis. 1980 v. 24 pp. 91−96.

117. Schwartz J. S. Pneumococcal vaccine: clinical efficacy and effectiveness. // Ann. Item. Med. 1982 v. 96 pp. 208−220;

118. Signas C., Akusjarvi G., Petterson U. Adenovirus 3 fiber polypeptide gene: implication for the structure of the fiber protein. // J. Virol. 1985 v. 53 № 3 pp. 67 267 8;

119. Silim A., Venne D. Comparison of egg-yolk and serum antibody titers to four avian viruses by enzyme-linked immunosorbent assay using paired field samples. //Avian. Diseases 1989 v. 33 pp. 643−648;

120. Smith K. A. Interleukin-2: inception, impact, and implications. //Science 1988 May 27 v. 240(4856)pp. 116 976;

121. Smith J. A., McFerran J. B. Egg drop syndrome// Prog. Vet. Microbiol. Immunol. Basel, Kargor, 1989 № 3 pp. 83−103;

122. Snyder D. G., Marguardt W. W., Mallinson E. T, Russel E. Enzyme-linked Immunosorbent Assay. 1. Measurment of antibody Activity Titer Against Newcastle Disease Virus in a Single Serum Dilution. //Avian. Diseases 1983 v. 27 pp. 161−170;

123. Stevenson S. C., Rollence M., White B., Weaver L., McClelland. Human adenovirus serotypes 3 and 5 bind to two different cellular receptors via the fiber head domain. // J. Virology 1995 pp. 2850−2857;

124. Stewart P. L., Chiu C. Y., Huang S., et all. Cryo-EM visualization of an exposed RGD epitope on adenovirus that escapes antibody neutralization. // EMBO J. 1997 Mar 17 v. 16(6)pp. 1189−98;

125. Suto R, Srivastava PK A mechanism for thespecific immunogenicity of heat shock protein-chaperoned peptides. // Science 1995 Sep. 15 v. 269(5230)pp. 1585−8;

126. Tang D. C., DeVit M., Johnston S. A. Genetic immunization is a simple method for eliciting an immune response. // Nature 1992 Mar 12 v. 356(6365)pp. 152−4;

127. Todd D., MoNulty M. S. Biochemical studies on a virus associated with egg drop syndrome 1976 // J. Gen. Virol. -1978 v. 40 pp. 63−67;

128. Tsukamoto K., Matsumura F., Mase M, I mai K. A highly sensitive, broadspectrum infectivity assay for infectious bursal disease virus. //Avian Diseases 1995 v. 39 pp. 575−586;

129. Ulmer J. B., Deck R. R., Dewitt C. M., Fu T. M., Donelly J. J. et all. Expression of a viral proteins by muscle cells in vivo induces protective cell-mediates immunity. //1997 Vaccine v. 15 pp. 839−841;

130. Van Raaij M. J., Louis N., Chroboczej J., Cusack S. Structure of the Human Adenovir7us Serotype 2 Fiber Head Domain at 1. 5A Resolution. // Virology 1999 v. 262 pp. 333−343;

131. Wang B., Boyer J. D., SrikantanV., Ugen K. et all. Induction of humoral and cellular immune responses to the human immunideficiency type 1 virus in nonhuman primates by in vivo DNA inoculation. //Virology 1995 v 211 pp. 102−112;

132. Wang B., Ugen K. E., Srikantan V. Et all. Gene Inoculation Generates Immune Responses Against Human Immunodeficiency Virus Type 1. //Proc. Natl. Acad. Sci 1993 v. 90 pp. 4156−4160;

133. Wang R., Doolan D. L., Thonge T. P., et all. Induction of antigen-specific cytoxic T lymphocytes in human by malaria DNA vaccine. // Science 1998 p. 47 6;

134. Watanabe A., Raz E., Kohsaka H., Tighe H., Barid S. M. et all. Induction of antibodies to a kappa region by gene immunization. // J. Immunol. 1993 v 151 pp. 28 712 8 7 6 ;

135. Watson G, Burdon M. G., Russell W. C. An antigenic analysis of the adenovirus type 2 fibre polypeptide. //J. Cen. Virol 1988 v. 69 pp. 525−535;

136. Wickham T. J., Lee G. M., Titus J. A. et all. Targeted adenovirus-mediated gene delivery to T cells via CD3. //J. Virology 1997 pp. 7663−7669;

137. Wigand R., Adrian T. Classification and epidemiology of adenoviruses // Adenovirus DNA / Ed. Doerfler W. Boston: M. Vijhoff Publ. -1986. -P. 409−441;

138. Wigand R., Bartha A., Dreizin R. S. et al. Adenoviridae: Second report // Intervirology. -1982. Vol. 18. -pp. 169;

139. Wigand R., Adrian T. Classification and epidemiology of adenoviruses // Adenovirus DNA / Ed. Doerfler W. Boston: M. Vijhoff Publ. -1986. -pp. 409−441;

140. Whalen R. G., Leclerc C., Deriaud E., Schirmbeck R., Reimann J., Davis H. L. DNA-mediated immunization to the hepatitis B surface antigen. Activation and entrainment of the immune response. // Ann. NY. Acad. Sei. 1995 Nov 27 v. 772 pp. 64−76;

141. Whitton J. L., Rodriguez F., Zhang J, Hassett D. E. DNA immunization: mechanistic studies. //Vaccine 1999 pp. 1612−1619;

142. Xia D., Henry L. J., Gerard R. D., Deisenhofer J. Crystal structure of the receptor-binding domain of adenovirus type '2 fiber protein at 1. 7A resolution. // Curr. Biol. Struct. 1994 v2 pp. 1259−1270;

143. Xiang Z. Q, Spitalnik S. L, Cheng J, et all. Immune responses to nucleic acid vaccines to rabies virus. Virology 1995 Jun 1 v. 209(2)pp. 569−79;

144. Xiang Z. Q., Spitalnik S., Tran M., et all. Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus. //Virology 1994 Feb 15v. 199(l)pp. 132−40;

145. Xu Z. Z., Both G. W. Altered tropism of an ovine adenovirus carrying the fiber protein cell binding domain of human adenovirus type 5. //Virology 1998 v. 248 pp. 156−163;

146. Yasue H., Ishibashi M. The oncogenicity of avian adenoviruses. III. In situ DNA hybridization of tumor line cells localized a large number of a virocellular sequence in few chromosomes. // Virology 1982 Jan 15 v. 116(l)pp. 99−115;

147. Yamaguchi S., Imada T., Kawamura H., Taniguchi T. et ¦ all. Outbreaks of egg drop syndrome 1976 in Japan and its etiological agent. //Avian. Diseases 1981 v. 25 № 3 pp. 642−649;

148. Yamamoto S., Yamamoto T., Kataoka T. et all. U nique palindromic sequences in syntetic oligonucleotides are required to induce IFN and augment IFN-mediated natiral killer activity. //J. Immunol. 1992 pp. 40 724 07 6;

149. Yokoyama M., Zhang J., Whitton J. L. DNA immunization confers protection against lethal lymphocytic choriomeningitis virus infection. //J. Virology 1995 v. 69 pp. 2684−2688;

150. Zakharchuk N., Kruglyak V. A., Akopian T. A., Naroditsky B. S. Tihonenko T. I Physical mapping and homology studies of egg drop syndrome (EDS-76)adenovirus DNA. //Arch. Virol 1993 v. 128 pp. 171−176;

151. Zanella A., DiDonato A., Nigrelli A., Poli G. Egg drop syndrome (1976): etiopathogenesis, epidemiology, imunology and control of the disease. // Clin. Vet. 1980 v. 103 № 7 pp. 459−469;

152. Zanella A., Poli A., Bentoni V. // Reserche preliminary sulla riposita iimunitaria alia vaccinazione con vaccino in veicolo oleoso contro la cossideta «Sindrome calo di derosizione-76». //Selezione Suinavicunicola 1978 Insert 39;

153. Zhu N., Liggitt D., Liu Y., Debs R. Systemic gene expression after intravenous DNA delivery into adult mice. // Science 1993 Jul 9 v. 261(5118)pp. 209−11;

154. Zsak L, Kisary J. Some biological and physico-chemical properties of egg drop syndrome (EDS)avian adenovirus strain B8/78. // Arch Virol 1981 v. 68(3−4)pp. 211−9;

155. Zsak L., Kissary J. Some biological and physico-chemical properties of egg drop syndrome (EDS)avian adenovirus strain B8/78. //Arch. Virol 1981 v6. 8 pp. 1219;

156. Zsak L., Szekely A., Kissary J. Experimental infection of joung and laying geese with egg drop syndrome -76 adenovirus stain B8/78. //Avian. Pathol 1982 v. 11 pp. 55 5−562;

157. Zsak L, Kisary J. Grouping of fowl adenoviruses based upon the restriction patterns of DNA generated by BamHI and Hindlll. // Intervirology 1984 v. 22(2)pp. 110.