ΠŸΠΎΠΌΠΎΡ‰ΡŒ Π² написании студСнчСских Ρ€Π°Π±ΠΎΡ‚
АнтистрСссовый сСрвис

ΠšΠΎΠ½ΡΡ‚Ρ€ΡƒΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈ ΠΈΠ·ΡƒΡ‡Π΅Π½ΠΈΠ΅ Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½Ρ‚Π½Ρ‹Ρ… адСновирусов CELO Π² экспСримСнтах in vitro ΠΈ in vivo

Π”ΠΈΡΡΠ΅Ρ€Ρ‚Π°Ρ†ΠΈΡΠŸΠΎΠΌΠΎΡ‰ΡŒ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈΠ£Π·Π½Π°Ρ‚ΡŒ ΡΡ‚ΠΎΠΈΠΌΠΎΡΡ‚ΡŒΠΌΠΎΠ΅ΠΉ Ρ€Π°Π±ΠΎΡ‚Ρ‹

Miyazawa N, Leopold PL, Hackett NR, Ferris B, Worgall S, Falck-Pedersen E, Crystal RG. Fiber swap between adenovirus subgroups B and C alters intracellular trafficking of adenovirus gene transfer vectors. // J. Virol., 1999, v.- 73(7), pp.- 6056βˆ’6065. Mayr GA, O’Donnell V, Chinsangaram J, Mason PW, Grubman MJ. Immune responses and protection against foot-and-mouth disease virus (FMDV) challenge… Π§ΠΈΡ‚Π°Ρ‚ΡŒ Π΅Ρ‰Ρ‘ >

ΠšΠΎΠ½ΡΡ‚Ρ€ΡƒΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈ ΠΈΠ·ΡƒΡ‡Π΅Π½ΠΈΠ΅ Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½Ρ‚Π½Ρ‹Ρ… адСновирусов CELO Π² экспСримСнтах in vitro ΠΈ in vivo (Ρ€Π΅Ρ„Π΅Ρ€Π°Ρ‚, курсовая, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½Ρ‚Ρ€ΠΎΠ»ΡŒΠ½Π°Ρ)

Π‘ΠΎΠ΄Π΅Ρ€ΠΆΠ°Π½ΠΈΠ΅

  • Π’Π«Π’ΠžΠ”Π«

1. Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½Π° ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠ° получСния Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½Ρ‚Π½Ρ‹Ρ… адСновирусов Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π³Π΅Π½ΠΎΠΌΠ° адСновируса ΠΏΡ‚ΠΈΡ† CELO, основанная Π½Π° Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡ‡Π½ΠΎΠΉ Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Ρ†ΠΈΠΈ Ρ„Ρ€Π°Π³ΠΌΠ΅Π½Ρ‚ΠΎΠ² вирусного Π³Π΅Π½ΠΎΠΌΠ° Π² ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… LMH. ΠžΡ‚Ρ€Π°Π±ΠΎΡ‚Π°Π½Ρ‹ ΠΎΠΏΡ‚ΠΈΠΌΠ°Π»ΡŒΠ½Ρ‹Π΅ условия получСния ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚ΠΈΠ²Π½Ρ‹Ρ… количСств Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½Ρ‚Π½ΠΎΠ³ΠΎ адСновируса ΠΏΡ‚ΠΈΡ† CELO Π² ΠΊΡƒΡ€ΠΈΠ½Ρ‹Ρ… эмбрионах. Π˜Π½Ρ„Π΅ΠΊΡ†ΠΈΠΎΠ½Π½Ρ‹ΠΉ Ρ‚ΠΈΡ‚Ρ€ ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚ΠΎΠ² вируса послС концСнтрирования ΠΈ ΠΎΡ‡ΠΈΡΡ‚ΠΊΠΈ составил 1011 Π‘ΠžΠ•/ΠΌΠ».

2. ΠŸΠΎΠ»ΡƒΡ‡Π΅Π½ Π½Π°Π±ΠΎΡ€ Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½Ρ‚Π½Ρ‹Ρ… адСновирусов ΠΏΡ‚ΠΈΡ† CELO, содСрТащих Ρ€Π΅ΠΏΠΎΡ€Ρ‚Π΅Ρ€Π½Ρ‹ΠΉ Π³Π΅Π½ GFP, Π° Ρ‚Π°ΠΊΠΆΠ΅ Π³Π΅Π½Ρ‹ Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ ΠΈ Π΄Π΅Ρ„Π΅ΠΊΡ‚Π½ΠΎΠ³ΠΎ Ρ€ΠΈΠ±ΠΎΠ·ΠΈΠΌΠΎΠ², Π½Π°ΠΏΡ€Π°Π²Π»Π΅Π½Π½Ρ‹Ρ… Π½Π° Ρ€Π°ΡΡ‰Π΅ΠΏΠ»Π΅Π½ΠΈΠ΅ мРНК Π³Π΅Π½Π° Π Π’1 вируса Π³Ρ€ΠΈΠΏΠΏΠ° А.

3. Π’ΠΏΠ΅Ρ€Π²Ρ‹Π΅ Π±Ρ‹Π»Π° ΠΏΠΎΠΊΠ°Π·Π°Π½Π° трансдукция Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½Ρ‚Π½Ρ‹ΠΌ адСновирусом ΠΏΡ‚ΠΈΡ† CELO-GFP ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€ ΠΊΠ»Π΅Ρ‚ΠΎΠΊ Ρ‡Π΅Π»ΠΎΠ²Π΅ΠΊΠ° Π»ΠΈΠ½ΠΈΠΉ Н1299, НСрЗВ ΠΈ ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€ ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ… Π»ΠΈΠ½ΠΈΠΉ MDBK, MDCK, COSI, RAT2, Π’16.

4. Π’ ΡΠΊΡΠΏΠ΅Ρ€ΠΈΠΌΠ΅Π½Ρ‚Π°Ρ… Π½Π° Π»Π°Π±ΠΎΡ€Π°Ρ‚ΠΎΡ€Π½Ρ‹Ρ… ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ… ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, Ρ‡Ρ‚ΠΎ Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ Π² Ρ…Π²ΠΎΡΡ‚ΠΎΠ²ΡƒΡŽ Π²Π΅Π½Ρƒ ΠΌΡ‹ΡˆΠΈ Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½Ρ‚Π½ΠΎΠ³ΠΎ адСновируса CELO-GFP ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ ΠΊ ΡΠΊΡΠΏΡ€Π΅ΡΡΠΈΠΈ Π³Π΅Π½Π° GFP Π² ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… ΠΏΠ΅Ρ‡Π΅Π½ΠΈ- Π²ΠΏΠ΅Ρ€Π²Ρ‹Π΅ ΠΏΠΎΠΊΠ°Π·Π°Π½Π° трансдукция ΠΊΠ»Π΅Ρ‚ΠΎΠΊ Π’16 ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ‹ Ρƒ ΠΌΡ‹ΡˆΠ΅ΠΉ ΠΏΡ€ΠΈ Π²Π½ΡƒΡ‚Ρ€ΠΈΠΎΠΏΡƒΡ…ΠΎΠ»Π΅Π²ΠΎΠΌ Π²Π²Π΅Π΄Π΅Π½ΠΈΠΈ Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½Ρ‚Π½ΠΎΠ³ΠΎ вируса CELO-GFP.

5. Π’ΠΏΠ΅Ρ€Π²Ρ‹Π΅ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ€Π΅ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ вируса Π³Ρ€ΠΈΠΏΠΏΠ°, А ΡˆΡ‚Π°ΠΌΠΌΠ° A/WSN/33 Π² ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… Π»ΠΈΠ½ΠΈΠΈ А54 9, ΠΈΠ½Ρ„ΠΈΡ†ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… Ρ€Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½Ρ‚Π½Ρ‹ΠΌ адСновирусом ΠΏΡ‚ΠΈΡ† CEL04D-N, ΡΠΊΡΠΏΡ€Π΅ΡΡΠΈΡ€ΡƒΡŽΡ‰ΠΈΠΌ Π³Π΅Π½ Ρ€ΠΈΠ±ΠΎΠ·ΠΈΠΌΠ°, Π½Π°ΠΏΡ€Π°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ Π½Π° Ρ€Π°ΡΡ‰Π΅ΠΏΠ»Π΅Π½ΠΈΠ΅ мРНК Π³Π΅Π½Π° Π Π’1 вируса Π³Ρ€ΠΈΠΏΠΏΠ° А. Π£Ρ€ΠΎΠ²Π΅Π½ΡŒ подавлСния Ρ€Π΅ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ вируса Π³Ρ€ΠΈΠΏΠΏΠ° составил 61,5%.

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