Бактериофаги и биосорбенты на их основе для специфической детекции клеток Escherichia coli биолюминесцентным методом

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1. Изучено взаимодействие бактерий E. coli В в растворе с бактериофагом Т4 и его рекомбинантными аналогами: фагом Т4, слитым с биотин связывающим белком (Т4-ВССР) и фагом Т4, слитым с доменом целлюлозосвязывающего белка (Т4-CBD). Показано, что литическая активность генетически модифицированных Т4-фагов ниже, чем исходного бактериофага Т4.

2. Получены биосорбенты путём иммобилизации фага. Т4-ВССР на магнитных стрептавидиновых частицах, и фага T4-CBD на! целлюлозных частицах. Показана высокая специфичность связывания рекомбинантных Т4-ВССР и T4-CBD фагов fс носителями, подтверждена прочность связывания.

3. Показано наличие более высокой литической активности биосорбентов на основе рекомбинантных бактериофагов по сравнению с аналогичными биосорбентами па основе исходного бактериофага Т4. Инфекционная активность иммобилизованных фагов снижена по сравнению с фагами в растворе.

4. Впервые нанофильтры «Disruptor» использованы для получения биосорбентов на основе бактериофага Т4 с целью задержания (на 99,9%) и"детекции бактерий E. coli В. Предел обнаружения при использовании метода с неиммобилизованным бактериофагом составил 500 КОЕ/мл, при> использовании метода с Т4-фагом, иммобилизованным на нанофильтре, — 730 КОЕ/мл. Показана высокая специфичность детекции клеток E. coli в смеси с культурой Salmonella Typhimurium.

5. Изучено взаимодействие эпителиальных HeLa клеток со светящимися клетками Е. coli, содержащими полный IwcCDABE оперон бактериальной люциферазы (15 штаммов, из которых 5 — непатогенные штаммы Е. coli, 10 — энтерогеморрагические штаммы (ЕНЕС), из них 5 — штаммы Е. coli 0157: Н7 и 5 — штаммы Е. coli других 0: Н серотипов). Впервые показано, что HeLa клетки активно способствуют росту различных штаммов Е coli, что может указывать! на важную регуляторную роль HeLa клеток в процессе колонизации эпителия бактериями.

6. Исследование адгезивной способности клеток E. coli к эпителиальным IleLa клеткам показало отсутствие зависимости адгезивной способности клеток E. coli от их вирулентных свойств. Характер поведения клеток Е coli в присутствии HeLa клеток был специфичен для каждого штамма.

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