Идентификация генов человека, экспрессия которых меняется при инфекции вирусом клещевого энцефалита

выводы.

1. Проведено сравнение тотальных препаратов РНК клеток человека почечной линии RH из инфицированных и неинфицированных вирусом клещевого энцефалита (ВКЭ), принадлежащего к роду Flavivirus семейства Flaviviridae. Обнаружен ряд последовательностей (в том числе, с использованием разработанной методики), супрессируемых и индуцированных в ходе инфекции, гомологичных известным генам.

2. Проведено доказательство их дифференциальной экспрессии альтернативными методами.

3. Впервые продемонстрирована индукция генов IFI-54K и IFI-56K при взаимодействии ВКЭ с клетками человека. Показан очень поздний характер индукции.

4. Разработана методика дифференциального скрининга полученных после супрессионной вычитающей гибридизации библиотек кДНК и создания высокообогащенных или упрощенных библиотек на их основе. Методика повышает воспроизводимость, эффективность и экономичность гибридизационного скрининга. На модельных системах показано, что методика позволяет снизить количество фоновых молекул в результирующей библиотеке, вплоть до практически полного их исчезновения. Эффективность методики продемонстрирована обнаружением дифференциальной экспрессии гена ADRP.

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